MLH1 Antibody

Datasheet
Code CSB-PA003247
Size US$167
Uniprot No. P40692
Image
  • Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody
  • Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody
Protocols Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Immunogen Synthesized peptide derived from the Internal region of Human MLH1.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications WB,IF,ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/20000
Form Liquid
Conjugate Non-conjugated
Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Alias MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
Immunogen Species Homo sapiens (Human)
Target Names MLH1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Involvement in disease Hereditary non-polyposis colorectal cancer 2 (HNPCC2); Mismatch repair cancer syndrome (MMRCS); Muir-Torre syndrome (MRTES); Endometrial cancer (ENDMC); Colorectal cancer (CRC)
Subcellular Location Nucleus
Protein Families DNA mismatch repair MutL/HexB family
Tissue Specificity Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Database Links

HGNC: 7127

OMIM: 114500

KEGG: hsa:4292

STRING: 9606.ENSP00000231790

UniGene: Hs.195364

Pathway DNA repair pathway

Related Products

MLH1 Antibodies

MLH1 Antibodies for Homo sapiens (Human)

MLH1 Antibodies for Human

MLH1 Proteins

MLH1 Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.