MPC2 Antibody

Datasheet

Code	CSB-PA002816LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: 293T whole cell lysate(30µg), PC-3 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg) All lanes: MPC2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 14 kDa Observed band size: 19 kDa Exposure time: 120s IHC image of CSB-PA002816LA01HU diluted at 1:50 and staining in paraffin-embedded mouse heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA002816LA01HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of HepG2 cell with CSB-PA002816LA01HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of PC-3 cell with CSB-PA002816LA01HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MPC2 Polyclonal antibody

Uniprot No.

O95563

Target Names

MPC2

Alternative Names

MPC2; BRP44; Mitochondrial pyruvate carrier 2; Brain protein 44

Raised in

Rabbit

Species Reactivity

Human

Immunogen

synthesized peptide derived from human MPC2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MPC2 Antibody (Product code: CSB-PA002816LA01HU) is Non-conjugated. For MPC2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002816LB01HU	MPC2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA002816LC01HU	MPC2 Antibody, FITC conjugated
Biotin	CSB-PA002816LD01HU	MPC2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Mediates the uptake of pyruvate into mitochondria.

Gene References into Functions

Hypoxia induces lactate secretion and glycolytic efflux by downregulating MPC1/MPC2 levels in HUVEC cells. PMID: 29845198
Our study indicates that MPC1 and MPC2 expressions are of prognostic values in PCAs and that positive expression of MPC1 or MPC2 is a predictor of favorable outcome. PMID: 27852261
a significant association between MPC2 variant rs10489202 and Schizophrenia susceptibility in Han Chinese (Meta-Analysis) PMID: 27460766
Results indicate mitochondrial pyruvate transporter (MPC) to be the key regulatory junction perturbed by virulent strains of Mycobacterium tuberculosis leading to alteration of mitochondrial metabolic flux and regulation of acetyl-CoA formation. PMID: 28263840
Tumor cells expressing MPC1 and MPC2 display increased mitochondrial pyruvate oxidation, with no changes in cell growth in adherent culture. PMID: 25458841
found no significant difference (P > 0.05) in either allele or genotype frequency in the SNPs between patients and controls. PMID: 23933155
characterization of the rat ortholog PMID: 3022128

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Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Protein Families

Mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family

Database Links

HGNC: 24515

OMIM: 614737

KEGG: hsa:25874

STRING: 9606.ENSP00000271373

UniGene: Hs.517768

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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