MRE11A Antibody

Code CSB-PA003273
Size US$167
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  • Western Blot analysis of COS7 cells using MRE11 Polyclonal Antibody
  • Western Blot analysis of K562 cells using MRE11 Polyclonal Antibody
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Product Details

Uniprot No. P49959
Target Names MRE11
Alternative Names AT like disease antibody; Ataxia telangiectasia disorder like antibody; ATLD antibody; DNA recombination and repair protein antibody; Double strand break repair protein MRE11A antibody; Double-strand break repair protein MRE11A antibody; endo/exonuclease Mre11 antibody; HNGS1 antibody; meiotic recombination (S. cerevisiae) 11 homolog A antibody; Meiotic recombination 11 homolog 1 antibody; meiotic recombination 11 homolog A (S. cerevisiae) antibody; Meiotic recombination 11 homolog A antibody; MmMRE11A antibody; Mre 11 antibody; MRE 11a antibody; MRE 11b antibody; MRE11 homolog 1 antibody; MRE11 homolog A antibody; MRE11 homolog double strand break repair nuclease antibody; MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody; MRE11 meiotic recombination 11 homolog A antibody; MRE11_HUMAN antibody; MRE11A antibody; MRE11b antibody; OTTHUMP00000236830 antibody; OTTHUMP00000236831 antibody; OTTHUMP00000236832 antibody; OTTHUMP00000236833 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat,Monkey
Immunogen Synthesized peptide derived from Human MRE11 around the non-phosphorylation site of S264.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Quinacrine based gold hybrid nanoparticle caused apoptosis through modulating replication fork in oral cancer stem cells. KC Hembram,Mol. Pharm,2020

Applications: Western Blot
Review: We checked the expression of BRCA-2 post NP treatment for different time periods (48, 72 and 96 h). It was noted that no significant change in BRCA-2 expression was found whereas RAD-51 level increased significantly (up to 3 fold in 96 h).
PMID: 32407635

Target Data

Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point
Gene References into Functions
  1. The effects of chronic smoking on oral mucosa led to the methylation of genes MRE11A PMS2, XRCC1 and MLH3, but resulted in a reduction of gene expression of MRE11A and PMS2, which showed >/=50% methylation. These results provide evidence that smoking cause methylation and reduced expression of repair genes. PMID: 29775861
  2. This study confirms that H2AFX variants are associated with an increased risk of BC. The above-reported sequence variants of MRE11 genes may not constitute a risk factor of breast cancer in the Polish population. PMID: 29678143
  3. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. PMID: 29651020
  4. Expression levels of MRN complex proteins(MRE11/RAD50/NBS1) significantly predict disease-free survival in rectal cancer patients, including those treated with neoadjuvant radiotherapy, and may have value in the management of these patients. PMID: 30176843
  5. Study proposes a new mechanism by which loss of PTEN and consequent activation of the PI3K-AKT-mTORC1-S6K1 signalling pathway impairs DNA repair by downregulation of MRE11. PMID: 28967905
  6. ATM-dependent phosphorylation of CtIP and the epistatic and coordinated actions of MRE11 and CtIP nuclease activities are required to limit the stable loading of Ku on single-ended DNA double-strand breaks. PMID: 27641979
  7. These evidences suggest that NBS1 is regulated by two kind of mechanisms: complex formation dependent on ATM, and protein degradation mediated by an unknown MG132-resistant pathway. PMID: 28369484
  8. MRE11A gene polymorphism is associated with colorectal cancer. PMID: 26735576
  9. Low MRE11 expression is associated with low-grade epithelial ovarian cancer. PMID: 28073364
  10. although recruitment of the MRE11-RAD50-NBS1 (MRN) DSB-sensing complex to viral genomes and activation of the ATM kinase can promote KSHV replication, proteins involved in nonhomologous end joining (NHEJ) repair restrict amplification of viral DNA. PMID: 28855246
  11. Mre11-Rad50-Nbs1 complex initiates DNA double strand break repair. PMID: 28867292
  12. we show that Plk1 phosphorylates Mre11 at S649 during G2 DNA damage recovery and Mre11 phosphorylation at S649/S689 drives premature checkpoint termination and reduced DNA repair PMID: 28512243
  13. In the absence of RAD51, the unprotected newly replicated genome is degraded by the exonuclease activity of MRE11, and the fragmented nascent DNA accumulates in the cytosol, initiating an innate immune response. PMID: 28334891
  14. Both the genome instability and cell death of MRE11-null and MRE11-mutated H129N cells are significantly reversed by overexpression of Tdp2, an enzyme that eliminates covalent Top2 conjugates; thus, the essential role of Mre11 nuclease activity is likely to remove the DNA lesions. PMID: 27814490
  15. The results illuminate the important role of Nbs1 and CtIP in determining the substrates and consequences of human Mre11/Rad50 nuclease activities on protein-DNA lesions. PMID: 27814491
  16. Cdk-dependent phosphorylation of TRF1 on threonine 371 promotes TRF1 to interact with APBs in S and G2 phases independently of its binding to telomeric DNA. We have demonstrated that the interaction of (pT371)TRF1 with APBs is dependent upon ATM and homologous-recombination-promoting factors such as Mre11 and BRCA1. PMID: 27185864
  17. Ataxia-telangiectasia-like disease (A-TLD) is clinically similar to mild Ataxia-telangiectasia and caused by hypomorphic mutations in the MRE11 gene. PMID: 27181190
  18. although Mre11 is required for efficient HR-dependent repair of ionizing-radiation-induced DSBs, Mre11 is largely dispensable for DSB resection in both chicken DT40 and human TK6 B cell lines. PMID: 27311583
  19. The aim of this study was to assess the interaction between MRE11 and clinicopathologic variables in breast cancer. PMID: 28133604
  20. The high expression of MRE11-RAD50-NBS1 complex constituents could be a predictor for poor prognosis and chemoresistance in gastric cancer PMID: 27798884
  21. The expression of DSB repair proteins such as RAD51 and MRE11 was investigated by immunohistochemistry, and associations between RAD51 and MRE11 expression and clinicopathological factors or chemotherapeutic effect were assessed PMID: 26676960
  22. The MRN complex is essential to restrain MYCN-induced replication stress during neural cell proliferation. PMID: 26068589
  23. This study found a significant trend indicating that the risk increases as the number of adverse alleles increase and significant three-locus interaction model involving NBS1 rs1805794, MRE11 rs10831234, and ATM rs227062. PMID: 26514363
  24. a significant increase in DKC1, RAD50, MRE11 and RPA1 expression in MM cases with high bone marrow infiltration (pPMID: 26366868
  25. Rad51 recombinase prevents Mre11 nuclease-dependent degradation and excessive PrimPol-mediated elongation of nascent DNA after UV irradiation PMID: 26627254
  26. The importance of the FGFR2-Mre11-DSBR link in cancer progression is suggested by the finding that genotypes of FGFR2 and Mre11 are associated with survival of breast cancer patients PMID: 25788520
  27. Furthermore, they collectively help to explain how MRN regulates DNA repair pathway choice. [review] PMID: 25576492
  28. Loss of the MRE11 protein predicts sensitivity to PARP-inhibitor sensitivity in vitro, defining it as an additional synthetic lethal gene with PARP. PMID: 24927325
  29. This work demonstrates that the Mre11-Rad50-Nbs1 DNA repair complex positively regulates AAV replication and plays a role in the integration of adeno-associated airus in the presence of herpes simplex virus 1. PMID: 25903339
  30. ATP switches the Mre11-Rad50-Nbs1 repair factor between signaling and processing of DNA ends. (Review) PMID: 25213441
  31. Low expression of MRE11 was associated with serous ovarian cancer. PMID: 24752797
  32. Analysis of this small number of colon cancer patients showed the dMRE11 marker predicts better prognosis independent of treatment in the long-term. In subgroup analyses, dMRE11 patients treated with irinotecan exhibited unexplained short-term mortality PMID: 25310185
  33. These data establish that MRE11A, RAD50, and NBN are intermediate-risk breast cancer susceptibility genes. PMID: 24894818
  34. These results articulate a model of inhibition of adeno-associated virus gene expression in which physical interaction of viral DNA with Mre11/Rad50/Nbs1 complex is more important than enzymatic activity. PMID: 25320294
  35. There was no correlation between bladder tumour MRE11 protein and RNA, suggesting MRE11 is controlled post-transcriptionally, a pattern confirmed in eight bladder cancer cell lines. PMID: 24625413
  36. This is the first report of somatic mutations within ESCO1 and CHTF18 in endometrial tumors and of MRE11A mutations in microsatellite-stable endometrial tumors. PMID: 23755103
  37. Germline MRE11A SNP rs1805363 was predictive of RT, but not of cystectomy outcome inMuscle-invasive bladder cancer . PMID: 24623370
  38. Data highlight a dual role for BLM that influences the DSB repair pathway choice: protection against CtIP/MRE11 long-range deletions associated with A-EJ and promotion of DNA resection. PMID: 24095737
  39. Microsatellite instability mutations were detected in MRE11 in myeloid malignancies conferring sensitivity to PARP inhibitors. PMID: 23349304
  40. The N-terminal mutations were found in ATLD patients with childhood cancer; thus, our studies suggest a clinically relevant dichotomy in MRE11A alleles PMID: 23912341
  41. Common genetic variations in the MRE11 and RAD50 genes do not contribute to an increased risk of laryngeal cancer. PMID: 24079363
  42. The formerly reported sequence variants in the RAD50 and MRE11 gene may not constitute a risk factor of childhood ALL in Polish population. PMID: 24093751
  43. Expression of cell cycle regulatory factors hus1, gadd45a, rb1, cdkn2a and mre11a correlates with expression of clock gene per2 in human colorectal carcinoma tissue. PMID: 24062075
  44. Rad17 is phosphorylated by ATM at Thr622 resulting in a direct interaction of Rad17 with NBS1, facilitating recruitment of MRE11, RAD50 and ATM to the DNA double-strand breaks. PMID: 24534091
  45. We found a significant relation between the expression of MRE11, NIBRIN and the postoperative survival of patients with pancreatic ductal adenocarcinoma PMID: 23954013
  46. DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. PMID: 24316220
  47. these data point to Mre11 as an important locus of Rsk-mediated checkpoint inhibition acting upstream of ATM activation. PMID: 24297933
  48. The Mre11-Rad50-Nbs1 (MRN) complex further stimulates resection in the presence of Ku and DNA-PKcs by recruiting Exo1 and enhancing DNA-PKcs autophosphorylation, and it also inhibits DNA ligase IV/XRCC4-mediated end rejoining. PMID: 24220101
  49. FANCJ helicase and MRE11 nuclease interact to facilitate the DNA damage response. PMID: 23530059
  50. Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex. PMID: 23525106
  51. TIS21 regulates DNA double strand break repair and apoptosis via increasing Mre11 methylation. PMID: 23089312
  52. MRE11 was shown to be a predictive marker for DSS after radiotherapy. * We have shown that TIP60 and MRE11 hold the potential to guide patients with invasive bladder cancer to either cystectomy or radiotherapy. PMID: 23046361
  53. High MRE11 expression in breast cancer cells was associated with more malignant behavior. MRE11 may be a novel oncoprotein & may serve as a new therapeutic target against breast cancer. PMID: 22914783
  54. Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses PMID: 22833675
  55. Mre11 and Nbs1 are sumoylated during Ad5 infection and the E4-ORF3 protein is necessary and sufficient to induce SUMO conjugation. Relocalization of Mre11 and Nbs1 into E4-ORF3 nuclear tracks is required for this modification to occur. PMID: 22740413
  56. Study identifies by whole-exome resequencing, mutations of MRE11, ZNF423, and CEP164 as causing Nephronophthisis-related ciliopathies. PMID: 22863007
  57. findings not only show that Mre11 activity is required for the survival of BRCA2 mutant cells but also elucidate roles for both the BRCA2 and PARP1 proteins in protecting stalled replication forks PMID: 22447567
  58. Review of the current literature suggests that hMRE11 is most likely involved with the MMR pathway in a more complex fashion than simply being a MMR target gene. PMID: 21849470
  59. Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex. PMID: 22179609
  60. The authors show that, in human and mouse, Mre11 controls these events through a direct interaction with CDK2 that is required for CtIP phosphorylation and BRCA1 interaction in normally dividing cells. PMID: 22231403
  61. Data demonstrate that DNA double-strand breaks activate signaling that promotes a PI3K-independent pathway of AKT phosphorylation that is dependent on MRE11-ATM-RNF168 signaling. PMID: 21623170
  62. The Mre11 structure in conjunction with biochemical analyses reveals that many tumorigenic mutations are primarily associated with Nbs1 binding and partly with nuclease activities PMID: 22078559
  63. these results showed a strong correlation between the MRE11A DNA repair gene (on both a SNP and a haplotype level) and gammaH2AX dephosphorylation PMID: 21880556
  64. findings implicate Mre11 as a target for heat radiosensitization and suggest that heat radiosensitization and inhibition of DSB repair may be mediated by heat-induced conformational changes in Mre11 PMID: 21699368
  65. BRCA2 prevents chromosomal aberrations on replication stalling, which are alleviated by inhibition of MRE11, the nuclease responsible for this form of fork instability. PMID: 21565612
  66. MSI colorectal tumors deficient in DSB repair secondary to mutation in MRE11 show a higher sensitivity to PARP-1 inhibition. PMID: 21300766
  67. two unrelated patients with Nijmegen breakage syndrome-like severe microcephaly PMID: 21227757
  68. The previously reported missense mutation W210C in MRE11 gene was identified in two families with autosomal recessive ataxia and oculomotor apraxia. PMID: 21324166
  69. BLM increases the affinity of EXO1 for ends, and MRN recruits and enhances the processivity of EXO1. PMID: 21325134
  70. we confirmed the important roles of Akt and Mre11 during the process of DNA double-strand break repair in cancer cells treated with ionizing radiation PMID: 20956948
  71. Mre11 nuclease plays a major role in initiating microhomology-mediated end joining in mammalian cells. PMID: 20647759
  72. this study shows significant differences in the levels of expression of MRN complex subunits, Mre11-Rad50-Nbs1, among various cancer cells and somatic cells. PMID: 20682289
  73. In this validated immunohistochemistry study, MRE11 protein expression was shown and confirmed as a predictive factor associated with survival following bladder cancer radiotherapy. PMID: 20843819
  74. The combination of MRE11 deficiency and DNA oxidative injury might have led to selective cerebellar degeneration PMID: 20087742
  75. Only mutation in the FHA domain, but not in the BRCT domains, yields a G2/M checkpoint defect, indicating that MDC1-dependent chromatin accumulation of the MRN complex at sites of DNA breaks is not required for G2/M checkpoint activation. PMID: 20224574
  76. haplotype-based tests identified significant associations between specific haplotypes in MRE11A and NBS1 genes and breast cancer risk PMID: 19714462
  77. summarise recent observation on MRN (composed of Mre11, Rad50 and Nbs1)structure, function, regulation and emerging mechanisms by which the MRN nano-machinery protects genomic integrity.[REVIEW] PMID: 19862546
  78. NASP and RCAS1 proteins were more frequently expressed in ovarian cancer tissues than with normal ovarian tissue and serous cystadenomas and MRE11 was less frequently expressed PMID: 20164540
  79. Plays role in the 3'-processing reaction and protection of the ends of viral DNA (HIV-1) after reverse transcription. Involved in multiple steps including integration and pre-integration steps during retroviral replication. PMID: 20003485
  80. 53BP1 promotes ATM activity through direct interactions with the MRN complex. PMID: 20010693
  81. MRE11-RAD50-NBS1 complex dictates DNA repair independent of H2AX. PMID: 19910469
  82. Major target for inactivation in mismatch repair-defective cells and may contribute to the development of colorectal cancer. PMID: 11850399
  83. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex PMID: 12124628
  84. activation in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes PMID: 12660252
  85. Mre11 complex is required for both ATM activation and the ATM-dependent G(2)/M checkpoint in response to double stranded breaks. PMID: 14657032
  86. WRN associates with the Mre11 complex via binding to Nbs1 in vitro and in vivo. PMID: 15026416
  87. Functional and structural characterization of a nuclease-deficient Mre11 protein termed mre11-3. PMID: 15047855
  88. MRE11 is a new common target in the mismatch repair deficient tumorigenesis and has a role in colorectal carcinogenesis PMID: 15048091
  89. Replication protein A, Mre11, Rad50 and Nbs1 bind and have roles in DNA repair PMID: 15180989
  90. Two hours at 41 degrees C did not increase the radiation sensitivity of cells with a reduced Mre11 protein level following a 24-h siRNA treatment. PMID: 15195510
  91. Nibrin, Mre11 and Rad50 also act as adaptors for some downstream Atm phosphorylation events PMID: 15234984
  92. MRE11 gene mutations affected ATM-dependent responses in radiosensitive ataxia-telangiectasia-like disorder family. PMID: 15269180
  93. MRE11 expression is impaired in gastric cancer with microsatellite instability PMID: 15319296
  94. Data show that Mre11 complex proteins are present in neurons of the adult human cortex and cerebellum, and suggest that loss of the complex may be associated with the pathogenesis of Alzheimer disease. PMID: 15337312
  95. early-onset, slowly progressive, ataxia plus ocular apraxia phenotype = ataxia telangiectasia-like disorder (ATLD) PMID: 15574463
  96. the Mre11/Rad50/Nbs1 (MRN) complex may play a more universal role in the recognition and response to DNA lesions of all types, whereas the role of RPA may be limited to certain subsets of lesions PMID: 15653682
  97. findings show that the Mre11-Rad50-Nbs1 (MRN) complex acts as a double-strand break sensor for ATM and recruits ATM to broken DNA molecules PMID: 15790808
  98. hMRE11 represents a functional component of the DNA mismatch repair pathway PMID: 15864295
  99. The MRE11-RAD50-NBS1 complex accelerates somatic hypermutation and gene conversion of immunoglobulin variable regions. PMID: 15937485
  100. These results suggest that MRE11 methylation regulates its association with nuclear structures such as PML nuclear bodies and sites of DNA damage. PMID: 15970667

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Involvement in disease Ataxia-telangiectasia-like disorder 1 (ATLD1)
Subcellular Location Nucleus, Chromosome, telomere
Protein Families MRE11/RAD32 family
Database Links

HGNC: 7230

OMIM: 600814

KEGG: hsa:4361

STRING: 9606.ENSP00000325863

UniGene: Hs.192649

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