MUC20 Antibody

Datasheet

Code	CSB-PA822693LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: MUC20 antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72, 69 kDa Observed band size: 72 kDa IHC image of CSB-PA822693LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA822693LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MUC20 Polyclonal antibody

Uniprot No.

Q8N307

Target Names

MUC20

Alternative Names

MUC 20 antibody; MUC-20 antibody; Muc20 antibody; MUC20_HUMAN antibody; Mucin 20 antibody; Mucin 20, cell surface associated antibody; Mucin-20 antibody; Transmembrane mucin MUC20S antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mucin-20 protein (569-657AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MUC20 Antibody (Product code: CSB-PA822693LA01HU) is Non-conjugated. For MUC20 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA822693LB01HU	MUC20 Antibody, HRP conjugated	ELISA
FITC	CSB-PA822693LC01HU	MUC20 Antibody, FITC conjugated
Biotin	CSB-PA822693LD01HU	MUC20 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.

Gene References into Functions

High MUC20 expression is associated wit response to chemotherapy in esophageal squamous cell carcinoma. PMID: 26673820
Results suggest that MUC20 enhances aggressive behaviors of EOC cells by activating integrin beta1 signaling and provide novel insights into the role of MUC20 in ovarian cancer metastasis. PMID: 26616226
MUC20 is a novel transmembrane mucin expressed by the human corneal and conjunctival epithelia, and suggest that differential expression of MUC20 during differentiation has a role in maintaining ocular surface homeostasis. PMID: 25168902
MUC20 is expressed in recurrent colorectal neoplasms and may have a role in poor outcome PMID: 23787019
Results suggest that MUC20 is a novel regulator of the Met signaling cascade which has a role in suppression of the Grb2-Ras pathway. PMID: 15314156

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Subcellular Location

Secreted. Apical cell membrane. Basolateral cell membrane. Cell projection, microvillus membrane.

Tissue Specificity

Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with th

Database Links

HGNC: 23282

OMIM: 610360

KEGG: hsa:200958

STRING: 9606.ENSP00000396774

UniGene: Hs.308992

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