MYH14 Antibody

Datasheet
Code CSB-PA773786LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q7Z406
Image
  • IHC image of CSB-PA773786LA01HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA773786LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA773786LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Myosin-14 protein (1058-1195AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
Relevance Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Myosin-14 (Myosin heavy chain 14) (Myosin heavy chain, non-muscle IIc) (Non-muscle myosin heavy chain IIc) (NMHC II-C), MYH14, KIAA2034
Immunogen Species Human
Research Area Signal Transduction
Target Names MYH14
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.
Involvement in disease Deafness, autosomal dominant, 4A (DFNA4A); Peripheral neuropathy, myopathy, hoarseness, and hearing loss (PNMHH)
Protein Families TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Tissue Specificity High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No
Database Links

HGNC: 23212

OMIM: 600652

KEGG: hsa:79784

STRING: 9606.ENSP00000262269

UniGene: Hs.467142

Pathway Regulation of actin cytoskeleton

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