Recombinant Human Myosin-14 (MYH14), partial

Code CSB-YP773786HU
MSDS
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Source Yeast
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Code CSB-EP773786HU
MSDS
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Source E.coli
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Code CSB-EP773786HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773786HU
MSDS
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Source Baculovirus
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Code CSB-MP773786HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYH14
Uniprot No.
Alternative Names
2400004E04Rik; DFNA4; DKFZp667A1311; FLJ13881; FLJ43092; FP17425; II C; KIAA2034; MHC16; Myh 14; MYH14; MYH14_HUMAN; Myosin 14; Myosin; Myosin heavy chain 14; Myosin heavy chain; Myosin heavy chain non muscle IIc; Myosin heavy polypeptide 14; Myosin-14; NMHC II C; NMHC II-C; Non muscle myosin heavy chain IIc; non-muscle IIc; Non-muscle myosin heavy chain IIc; Nonmuscle myosin heavy chain II C; OTTMUSP00000019210
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.
Gene References into Functions
  1. This is the first identification of mutations in MYH14 as a cause of Anorectal malformations. PMID: 28191911
  2. By reporting two novel variants of MYH14, we suggest that the present study extends the phenotypic spectrum of autosomal dominant MYH14 variants to include nonsyndromic, severe-to-profound hearing loss with prelingual onset. PMID: 28221712
  3. phosphorylation of human cardiac myosin regulatory light chain prevents development of hypertrophic cardiomyopathy in mice PMID: 26124132
  4. The data show that although NM IIA and IIB form filaments with similar properties, NM IIC forms filaments that are less well suited to roles such as tension maintenance within the cell. PMID: 24072716
  5. Results support the hypothesis that there is another DFNA gene upstream of the MYH14 gene and it may be linked to the DFNA4 locus. PMID: 23273769
  6. the alterations of the MYH14 gene may contribute to Myotonic dystrophy type 1 molecular pathogenesis. PMID: 21872659
  7. A complex phenotype of peripheral neuropathy, myopathy, hoarseness, and hearing loss is linked to an autosomal dominant mutation in MYH14 PMID: 21480433
  8. kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoforms PMID: 21478157
  9. data identify CEACAM16 as an alpha-tectorin-interacting protein that concentrates at the point of attachment of the TM to the stereocilia and, when mutated, results in ADNSHL at the DFNA4 locus PMID: 21368133
  10. Mutation in MYH14 gene can cause dominant non-syndromic hearing impairment in Asian population, suggesting that MYH14 gene can be a disease-causing gene of Chinese patients with hearing impairment. PMID: 20533261
  11. mutational screening in a large series of 300 hearing-impaired patients from Italy, Spain, and Belgium and in a German kindred linked to DFNA4 PMID: 15015131
  12. mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C PMID: 15845534
  13. data do not support the involvement of MYH14 in cleft lip and palate among the Italian population PMID: 18471249
  14. the alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation PMID: 19240025

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Involvement in disease
Deafness, autosomal dominant, 4A (DFNA4A); Peripheral neuropathy, myopathy, hoarseness, and hearing loss (PNMHH)
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Tissue Specificity
High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No
Database Links

HGNC: 23212

OMIM: 600652

KEGG: hsa:79784

STRING: 9606.ENSP00000262269

UniGene: Hs.467142

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