NADK2 Antibody

Code CSB-PA676825LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue
    All lanes: NADK2 antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 50, 46, 32 kDa
    Observed band size: 50, 46 kDa

  • IHC image of CSB-PA676825LA01HU diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA676825LA01HU diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NADK2 Polyclonal antibody
Uniprot No.
Target Names
NADK2
Alternative Names
C5orf33 antibody; CE033_HUMAN antibody; Chromosome 5 open reading frame 33 antibody; FLJ30596 antibody; Hypothetical protein LOC133686 antibody; MGC43298 antibody; NADKD1 antibody; OTTHUMP00000161732 antibody; UPF0465 protein C5orf33 antibody
Raised in
Rabbit
Species Reactivity
Human, Rat, Mouse
Immunogen
Recombinant Human NAD kinase 2, mitochondrial protein (16-156AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NADK2 Antibody (Product code: CSB-PA676825LA01HU) is Non-conjugated. For NADK2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA676825LB01HU NADK2 Antibody, HRP conjugated ELISA
FITC CSB-PA676825LC01HU NADK2 Antibody, FITC conjugated
Biotin CSB-PA676825LD01HU NADK2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.
Gene References into Functions
  1. The use of whole exome sequencing has allowed us to identify a new homozygous mutation in NADK2, leading to a premature stop codon and absence of protein. To our knowledge this is the second reported individual with mutations in NADK2. PMID: 27940755
  2. An autosomal recessive defect in NADK2 causes mitochondrial NADPH deficiency with secondary deficiencies in several mitochondrial processes including polyunsaturated fatty acid oxidation and lysine degradation. PMID: 24847004
  3. exhibits NAD kinase activity, utilizing ATP or inorganic polyphosphate, and is localized in mitochondria of HEK293A cells PMID: 23212377
  4. MNADK is localized to mitochondria in Hep G2 cells, and a recombinant MNADK showed NAD kinase activity. MNADK is abundant in mitochondrion-rich tissues, such as liver. MNADK expression was suppressed in human liver tumors. Identification of MNADK immediately suggests a model in which NADK and MNADK are responsible for de novo synthesis of NADP(+) in cytosol and mitochondria, respectively. PMID: 23616928
Involvement in disease
2,4-dienoyl-CoA reductase deficiency (DECRD)
Subcellular Location
Mitochondrion.
Protein Families
NAD kinase family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 26404

OMIM: 615787

KEGG: hsa:133686

STRING: 9606.ENSP00000371362

UniGene: Hs.81907

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