NCF2 Antibody, FITC conjugated

Code CSB-PA015528LC01HU
Size US$166
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NCF2 Polyclonal antibody
Uniprot No.
Target Names
NCF2
Alternative Names
67 kDa neutrophil oxidase factor antibody; Chronic granulomatous disease autosomal 2 antibody; FLJ93058 antibody; NADPH oxidase activator 2 antibody; NCF-2 antibody; Ncf2 antibody; NCF2_HUMAN antibody; Neutrophil cytosol factor 2 antibody; Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody; Neutrophil NADPH oxidase factor 2 antibody; NOXA2 antibody; P67 PHOX antibody; p67-phox antibody; p67phox antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Neutrophil cytosol factor 2 protein (178-526AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
FITC
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Gene References into Functions
  1. We analyzed the clinical and laboratory findings of CGD with mutations in the NCF2 gene from amongst our cohort of CGD patients. A homozygous mutation (c.835_836delAC, p.T279fsX294), a deletion in NCF2 gene was found in two cases. In the third case, two heterozygous mutations were detected, IVS13-2A>T on one allele and c.1099C>T (p.) on the other allele. PMID: 28035544
  2. All investigated patients presented the same mutation (c.257 + 2T > C) in NCF2 gene. We show that this mutation is responsible for a drastic decrease of p67phox mRNA and leads to the skipping of exon 3 detected in the low amount of residual mRNA. PMID: 27220316
  3. Phosphoinositol 3-phosphate regulates reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane. PMID: 28096301
  4. TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for reactive oxygen species generation, thus regulating IL-1beta transcription and processing in monocytic cells. PMID: 26320741
  5. Skeletal muscle protein expression of the NADPH oxidase subunits p22(phox), p47(phox), and p67(phox) was increased in obese relative to lean subjects, where p22(phox) and p67(phox) expression was attenuated by exercise training in obese subjects. PMID: 27765769
  6. A novel homozygous mutation in NCF2. PMID: 26272171
  7. results reveal an essential role for the Cys-Gly-Cys triad in Nox2 in binding p67(phox), seconded by an additional binding region, comprising residues C terminal to Cys-Gly-Cys. The 2 regions interact with distinct partner sites in p67(phox). PMID: 26160850
  8. This model assigns a central role to Arg-395 in the structure and stability of the quaternary NCF2/NCF4/VAV1/RAC1 NADPH oxidase complex. PMID: 25795782
  9. Data indicate that arachidonic acid induces the direct interaction of Rac-GTP-bound p67(phox) with the C-terminal cytosolic region of phagocyte NADPH oxidase Nox2. PMID: 25056956
  10. Four novel mutations in the NCF1, NCF2, and CYBB genees have been identified in chronic granulomatous disease patients in Morocco. PMID: 24596025
  11. Results not only establish allelic heterogeneity within NCF2 associated with SLE, but also emphasize the utility of multi-ethnic cohorts to identify predisposing variants explaining additional phenotypic variance of complex diseases like SLE. PMID: 24163247
  12. NCF2 in Asian populations shows a pattern of diversity characterized by a differentiated haplotype structure. PMID: 23821607
  13. Results provide insight into the redox-sensitive signaling mechanism that mediates cell survival involving p53 and its novel target NCF2/p67phox. PMID: 23187810
  14. Case Report: report defects in NCF-2, teh gene encoding p67-phox, in four cases of chronic granulomatous disease. PMID: 23264412
  15. Eight novel mutations in CYBB and NCF2 genes were identified in patients with chronic granulomatous disease. PMID: 22562447
  16. This variant reduced binding of the NCF2 gene product p67(phox) to RAC2. This study found a novel genetic association of RAC2 with Crohn's disease (CD) and replicated the previously reported association of NCF4 with ileal CD. PMID: 21900546
  17. NCF2 is strongly associated with increased risk of childhood- and adult-onset systemic lupus erythematosus through a single nonsynonymous coding mutation (H389Q) in exon 12. PMID: 22203994
  18. p67(phox) has a critical role to support for reactive oxygen species production on the level of individual phagosomes. PMID: 21954286
  19. Association analysis identified five SLE susceptibility genes reaching genome-wide levels of significance : NCF2 ,IKZF1 ,IRF8 ,IFIH1 , and TYK2 PMID: 22046141
  20. The genetic variation in the NCF2 gene was found to associate with SLE in US and European populations PMID: 20842512
  21. High NCF2 expression in the cytoplasm is associated with uterine cervix carcinogenesis. PMID: 21119665
  22. the extended activation domain of p67(phox) (amino acids 190-210) containing the D(Y/F)LGK motif plays an essential role in oxidase activation probably by interacting with gp91(phox). PMID: 20679349
  23. mutations in CYBB, NCF1, CYBA or NCF2 may play a role in chronic granulomatous disease PMID: 18546332
  24. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in idiopathic pulmonary fibrosis patients. PMID: 17651608
  25. All mutations and some polymorphisms identified in the NCF2 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
  26. Here we show that p67(phox)adopts an elongated conformation when it exists not only as a monomer but also as the heterotrimer PMID: 20375610
  27. These findings identify the activation of PKC delta and NADPH oxidase as crucial steps in retinoic acid-induced neuroblastoma cell differentiation. PMID: 20074641
  28. Alu-induced deletion of the TPR4 domain of p67-phox leads to loss of function and accelerated degradation of the protein. PMID: 19953534
  29. In a cell-free system, covalent binding between C-terminal-truncated p67phox and rac in the correct fusion order produces a more stable complex than the individual components and significantly influences the duration of fusion-produced oxidase activation. PMID: 11705402
  30. detailed study of the protein-protein interactions that occur in the p40-p47-p67(phox) complex of the resting oxidase PMID: 11796733
  31. p22(phox), gp91(phox), p47(phox), p67(phox), and p40(phox) existed as a functional complex in the cytoskeletal fraction. PMID: 11893732
  32. Val204 in p67(phox), previously shown to be required for NADPH oxidase activity under cell-free conditions, was found to be essential for superoxide production by intact COS-phox cells. PMID: 11929750
  33. effect of cPLA2 on its translocation PMID: 12101222
  34. NAD(P)H oxidase subunits p47(phox) and p67(phox) are expressed in platelets; and NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment. PMID: 12130503
  35. p67phox and p47phox have roles in regulating a change of conformation in cytochrome b558, which initiates the electron transfer in NADPH oxidase activation PMID: 12719414
  36. NOXO1, p47phox, and p67phox regulate Nox3 PMID: 15181005
  37. NAD(P)H oxidase activity is associated with increased protein levels of p22phox, p47phox, and p67phox, and increased p22phox and nox2 (gp91phox) mRNA expression. PMID: 15256399
  38. Increased expression and activity of NAD(P)H oxidase subunits and xanthine oxidase, in part mediated through angiotensin II and PKC-dependent pathways, are important mechanisms underlying increased oxidative stress in human coronary artery disease PMID: 16293794
  39. Here we show that the p47(phox)-p67(phox) interaction is disrupted not only by deletion of the PRR but also by substitution for basic residues in the extra-PRR (K383E/K385E). PMID: 16297854
  40. Expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation. PMID: 16310324
  41. These results indicate that Hcy (homocysteine)-stimulated superoxide anion production in monocytes is regulated through PKC-dependent phosphorylation of p47phox and p67phox subunits of NADPH oxidase. PMID: 16626305
  42. NADPH oxidase assembly from p67phox was studies at the single-cell level. PMID: 16987007
  43. chemoattractant-stimulated superoxide production can be amplified by a positive feedback loop in which p67(phox) targets Vav1-mediated Rac activation PMID: 17060455
  44. These data clearly identify PLAGL2 as a novel regulator of NCF2/p67phox gene expression as well as NADPH oxidase activity and contribute to a greater understanding of the transcriptional regulation of NCF2. PMID: 17462995
  45. a novel single nucleotide polymorphism in the promoter region PMID: 17712795
  46. Single nucleotide polymorphism leads to alternative splicing without altering gene expression or respiratory burst activity. PMID: 17910042
  47. p40(phox) translocates p67(phox) to the region of the cytochrome and subsequently switches the oxidase to an activated state dependent upon PtdIns(3)P and SH3 domain engagement. PMID: 18029359
  48. As(2)O(3) induced phosphorylation and membrane translocation of the NADPH oxidase subunit p47(phox) and it also increased translocation of Rac1 and p67(phox). PMID: 18424721
  49. autosomal recessive CGD due to NCF-2 gene mutations, and a novel homozygous and hypomorphic NCF-2 gene mutation was found. PMID: 18625437
  50. p67(phox)-SH3(N) specifically functions in gp91(phox)/Nox2 activation probably via facilitating oxidase assembly. PMID: 19116138

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Involvement in disease
Granulomatous disease, chronic, cytochrome-b-positive 2, autosomal recessive (CGD2)
Subcellular Location
Cytoplasm.
Protein Families
NCF2/NOXA1 family
Database Links

HGNC: 7661

OMIM: 233710

KEGG: hsa:4688

STRING: 9606.ENSP00000356505

UniGene: Hs.587558

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