Product Details

Uniprot No.

P19878

Target Names

Alternative Names

Neutrophil cytosol factor 2 (NCF-2) (67 kDa neutrophil oxidase factor) (NADPH oxidase activator 2) (Neutrophil NADPH oxidase factor 2) (p67-phox), NCF2, NOXA2 P67PHOX

Species Reactivity

Human

Immunogen

A synthesized peptide from human NCF2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9B8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NCF2, also known as p67-phox, serves as an essential cytosolic activator of the NADPH oxidase complex, playing a critical role in the respiratory burst that enables phagocytes to destroy invading pathogens. This protein's involvement in reactive oxygen species generation makes it a key focus for researchers investigating innate immunity, chronic granulomatous disease, and inflammatory conditions where oxidative stress contributes to pathology.

This recombinant monoclonal antibody, clone 9B8, offers the reproducibility that demanding research protocols require. Because the antibody sequence is defined and produced through recombinant technology, you can expect consistent performance across experiments and between lots—eliminating the variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format provides strong signal amplification while maintaining specificity for human NCF2.

Validation testing demonstrates this antibody's versatility across multiple experimental platforms. Immunohistochemistry studies using human liver cancer and lymph node tissues at 1:100 dilution show clear target detection following citrate buffer antigen retrieval, making it suitable for examining NCF2 expression patterns in both malignant and lymphoid tissue contexts. Flow cytometry analysis using Jurkat cells confirms the antibody's utility for quantifying intracellular NCF2 levels in T cell populations, with distinct separation between specific staining and isotype control observed at 1:100 dilution. The antibody is also validated for ELISA applications.

Whether you're characterizing NADPH oxidase assembly in immune cells, investigating oxidative stress mechanisms in cancer microenvironments, or studying inflammatory signaling pathways, this affinity-purified antibody provides a reliable tool for advancing your understanding of NCF2 biology across tissue-based and cell-based experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA991818A0HU
Size	US$210
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Image	IHC image of CSB-RA991818A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA991818A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA991818A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NCF2 Recombinant Monoclonal Antibody

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