NDUFB5 Antibody

Datasheet
Code CSB-PA015652ESR1HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: NDUFB5 antibody at 2.64µg/ml
    Lane 1: Mouse heart tissue
    Lane 2: Mouse kidney tissue
    Lane 3: 293T whole cell lysate
    Lane 4: Jurkat whole cell lysate
    Lane 5: MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 22, 16 kDa
    Observed band size: 16 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015652ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA015652ESR1HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA015652ESR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) NDUFB5 Polyclonal antibody
Uniprot No. O43674
Target Names NDUFB5
Alternative Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (Complex I-SGDH) (CI-SGDH) (NADH-ubiquinone oxidoreductase SGDH subunit), NDUFB5
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial protein (100-189AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

Potentially Critical Roles of NDUFB5, TIMMDC1, and VDAC3 in the Progression of Septic Cardiomyopathy Through Integrated Bioinformatics Analysis. Kang K, et al,Dna and Cell Biology,2019

Sample type: Heart tissue
Sample species: Mouse
Application:WB
Review: Expression of hub genes was measured by qRT- PCR and Western blotting. Scale bar: 20mm (A); mean–SEM value of at least six mice per group.
PMID: 31794266

Target Data

Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Subcellular Location Mitochondrion inner membrane, Single-pass membrane protein, Matrix side
Protein Families Complex I NDUFB5 subunit family
Database Links

HGNC: 7700

OMIM: 603841

KEGG: hsa:4711

STRING: 9606.ENSP00000259037

UniGene: Hs.730674

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