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NIN Antibody

Datasheet
Code CSB-PA843155LA01HU
Size US$166
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Image
  • Immunohistochemistry(IHC) 1- NIN Antibody

    Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA843155LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NIN Polyclonal antibody
Uniprot No.
Q8N4C6
Target Names
NIN
Alternative Names
Centrosome Marker antibody; Glycogen synthase kinase 3 beta interacting protein antibody; GSK3B interacting protein antibody; h Glycogen synthase kinase 3 beta-interacting protein antibody; hNinein antibody; KIAA1565 antibody; NIN antibody; NIN protein antibody; NIN_HUMAN antibody; ninein (GSK3B interacting protein) antibody; Ninein antibody; Ninein centrosomal protein antibody; SCKL7 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Ninein protein (80-185AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NIN Antibody (Product code: CSB-PA843155LA01HU) is Non-conjugated. For NIN Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA843155LB01HU NIN Antibody, HRP conjugated ELISA
FITC CSB-PA843155LC01HU NIN Antibody, FITC conjugated
Biotin CSB-PA843155LD01HU NIN Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Protocols
ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole.
Gene References into Functions
  1. Our analyses suggest a relationship with prenatal neurogenesis and identify the human data point as an outlier, possibly explained by postnatal changes in development on the human lineage. PMID: 22937743
  2. Ninein influences the rate of axonal growth and branching by affecting microtubule stability and dynamics. PMID: 25741725
  3. JAK2 Tyrosine Kinase phosphorylates centrosomal protein ninein, which negatively regulates it PMID: 25332239
  4. A Ninein (NIN) missense mutation identified in a family with spondyloepimetaphyseal dysplasia with joint laxity (leptodactylic type)-like phenotype. PMID: 23665482
  5. The role of hNinein isoform 6 expression in cell differentiation was assessed in BrdU-treated IMR-32 cells PMID: 23047184
  6. The prevalence of hNinein autoreactivity and its specificity in 22 rheumatoid arthritis and 32 systemic lupus erythematosus autoimmune disease sera. PMID: 21667027
  7. Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. PMID: 21325031
  8. Two single nucleotide polymorphisms in the APC and NIN loci were significantly associated with pancreatic cancer risk PMID: 20056645
  9. Ninein is essential for the reformation of specific aspects of the interphase centrosome architecture following mitosis as well as being required for the centrosome to function as a MTOC. PMID: 11956314
  10. ninein protein has two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle PMID: 12927815
  11. Elevated ninein causes mislocalization of gamma-tubulin, recruiting it to ectopic (noncentrosomal) ninein-containing sites which are not active in nucleating microtubules, suggesting a regulatory role for ninein in microtubule nucleation. PMID: 15190203
  12. We propose that ninein constitutes a molecular link between microtubule-nucleation and -anchoring activities at the centrosome. PMID: 15784680
  13. our findings place SUMOylation target on the centrosome structure protein, hNinein, which results in the switch localization from centrosome to nucleus PMID: 16154161
  14. analysis of human ninein isoforms that are regulated by centrosomal targeting signals and bind to gamma-tubulin PMID: 17102634
  15. hNinein regulates the dynamic movement of Astrin throughout the cell cycle and this interaction, in turn, is required for maintenance of centrosome/spindle pole integrity. PMID: 17383637
  16. Data show that ninein is highly dynamic and that, in epithelial cells, it is present not only at the centrosome but also in the cytoplasm as distinct speckles. PMID: 17698918

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Involvement in disease
Seckel syndrome 7 (SCKL7)
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.; [Isoform 6]: Cytoplasm.
Tissue Specificity
Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5.
Database Links

HGNC: 14906

OMIM: 608684

KEGG: hsa:51199

STRING: 9606.ENSP00000371472

UniGene: Hs.310429

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