NIT1 Antibody

Datasheet

Code	CSB-PA768228LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: NIT1 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36, 32, 41, 35, 27 kDa Observed band size: 36 kDa IHC image of CSB-PA768228LA01HU diluted at 1:1200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA768228LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NIT1 Polyclonal antibody

Uniprot No.

Q86X76

Target Names

NIT1

Alternative Names

MGC57670 antibody; NIT1 antibody; NIT1_HUMAN antibody; Nitrilase 1 antibody; Nitrilase homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Deaminated glutathione amidase protein (1-243AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NIT1 Antibody (Product code: CSB-PA768228LA01HU) is Non-conjugated. For NIT1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA768228LB01HU	NIT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA768228LC01HU	NIT1 Antibody, FITC conjugated
Biotin	CSB-PA768228LD01HU	NIT1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:1000-1:2000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.

Gene References into Functions

Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo. PMID: 26967383
Human NIT1/2 are not involved in the metabolism of vildagliptin. PMID: 25008847
Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578

Subcellular Location

[Isoform 2]: Mitochondrion.; [Isoform 1]: Cytoplasm.

Protein Families

Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family

Tissue Specificity

Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 7828

OMIM: 604618

KEGG: hsa:4817

STRING: 9606.ENSP00000356988

UniGene: Hs.146406

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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