NLN Antibody

Datasheet
Code CSB-PA863161LA01HU
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Size US$299
Uniprot No. Q9BYT8
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate
    All lanes: NLN antibody at 3.2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 81 kDa
    Observed band size: 81 kDa

  • IHC image of CSB-PA863161LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA863161LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Neurolysin, mitochondrial protein (109-241AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Neurolysin, mitochondrial (EC 3.4.24.16) (Angiotensin-binding protein) (Microsomal endopeptidase) (MEP) (Mitochondrial oligopeptidase M) (Neurotensin endopeptidase), NLN, AGTBP KIAA1226
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Tags & Cell Markers
Target Names NLN
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
Subcellular Location Mitochondrion intermembrane space, Cytoplasm
Protein Families Peptidase M3 family
Database Links

HGNC: 16058

OMIM: 611530

KEGG: hsa:57486

STRING: 9606.ENSP00000370372

UniGene: Hs.247460

Pathway Renin-angiotensin system

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