NLN Antibody

Datasheet

Code	CSB-PA863161LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate All lanes: NLN antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 81 kDa IHC image of CSB-PA863161LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA863161LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NLN Polyclonal antibody

Uniprot No.

Q9BYT8

Target Names

NLN

Alternative Names

AGTBP antibody; Angiotensin binding protein antibody; Angiotensin-binding protein antibody; DKFZp564F123 antibody; EC 3.4.24.16 antibody; EP24.16 antibody; FLJ23002 antibody; KIAA1226 antibody; MEP antibody; Microsomal endopeptidase antibody; mitochondrial antibody; Mitochondrial oligopeptidase M antibody; MOP antibody; NEUL_HUMAN antibody; neurolysin (metallopeptidase M3 family) antibody; Neurolysin antibody; Neurolysin, mitochondrial precursor antibody; Neurotensin endopeptidase antibody; Nln antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Neurolysin, mitochondrial protein (109-241AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NLN Antibody (Product code: CSB-PA863161LA01HU) is Non-conjugated. For NLN Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA863161LB01HU	NLN Antibody, HRP conjugated	ELISA
FITC	CSB-PA863161LC01HU	NLN Antibody, FITC conjugated
Biotin	CSB-PA863161LD01HU	NLN Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.

Gene References into Functions

Mutations at only two residues (Arg-470 and Arg-498) are required to swap specificity with thimet oligopeptidase, a result that is confirmed by testing the two-mutant constructs. PMID: 17251185

Subcellular Location

Mitochondrion intermembrane space. Cytoplasm.

Protein Families

Peptidase M3 family

Database Links

HGNC: 16058

OMIM: 611530

KEGG: hsa:57486

STRING: 9606.ENSP00000370372

UniGene: Hs.247460

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Organelles Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Renin-angiotensin system

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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