NLN Antibody

Code CSB-PA863161LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate
    All lanes: NLN antibody at 3.2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 81 kDa
    Observed band size: 81 kDa

  • IHC image of CSB-PA863161LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA863161LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NLN Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
AGTBP antibody; Angiotensin binding protein antibody; Angiotensin-binding protein antibody; DKFZp564F123 antibody; EC 3.4.24.16 antibody; EP24.16 antibody; FLJ23002 antibody; KIAA1226 antibody; MEP antibody; Microsomal endopeptidase antibody; mitochondrial antibody; Mitochondrial oligopeptidase M antibody; MOP antibody; NEUL_HUMAN antibody; neurolysin (metallopeptidase M3 family) antibody; Neurolysin antibody; Neurolysin, mitochondrial precursor antibody; Neurotensin endopeptidase antibody; Nln antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Neurolysin, mitochondrial protein (109-241AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NLN Antibody (Product code: CSB-PA863161LA01HU) is Non-conjugated. For NLN Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA863161LB01HU NLN Antibody, HRP conjugated ELISA
FITC CSB-PA863161LC01HU NLN Antibody, FITC conjugated
Biotin CSB-PA863161LD01HU NLN Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
Gene References into Functions
  1. Mutations at only two residues (Arg-470 and Arg-498) are required to swap specificity with thimet oligopeptidase, a result that is confirmed by testing the two-mutant constructs. PMID: 17251185
Subcellular Location
Mitochondrion intermembrane space. Cytoplasm.
Protein Families
Peptidase M3 family
Database Links

HGNC: 16058

OMIM: 611530

KEGG: hsa:57486

STRING: 9606.ENSP00000370372

UniGene: Hs.247460

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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