NRCAM Antibody

Datasheet

Code	CSB-PA016074LA01HU
Size	US$166
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Image	IHC image of CSB-PA016074LA01HU diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA016074LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of 293 cells with CSB-PA016074LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NRCAM Polyclonal antibody

Uniprot No.

Q92823

Target Names

NRCAM

Alternative Names

Bravo antibody; hBravo antibody; Neuronal cell adhesion molecule antibody; Neuronal surface protein Bravo antibody; Ng CAM related antibody; Ng-CAM-related antibody; NgCAM related cell adhesion molecule antibody; NgCAM-related cell adhesion molecule antibody; Nr CAM antibody; Nr-CAM antibody; Nrcam antibody; NRCAM_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Neuronal cell adhesion molecule protein (1194-1299AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NRCAM Antibody (Product code: CSB-PA016074LA01HU) is Non-conjugated. For NRCAM Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA016074LB01HU	NRCAM Antibody, HRP conjugated	ELISA
FITC	CSB-PA016074LC01HU	NRCAM Antibody, FITC conjugated
Biotin	CSB-PA016074LD01HU	NRCAM Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.

Gene References into Functions

Increased gene expression of NrCAM may be used as a potential diagnostic marker for papillary thyroid cancer. PMID: 27732334
A high level of Nr-CAM expression is associated with favorable tumor phenotype and reduced risk of prostate cancer recurrence. PMID: 23338556
Genome-wide association analysis of coffee drinking suggests association with CYP1A1/CYP1A2 and NRCAM PMID: 21876539
mechanisms of NrCAM participation in development and how these might be perturbed in disorders are reviewed. [review] PMID: 22182708
Nr-CAM protein expression is upregulated in colorectal cancer tissues; Nr-CAM overexpression is an independent marker of poor prognosis among advanced CRC patients, and a possible predictive marker for non-beneficence to 5-fluorouracil-based chemotherapy PMID: 21718388
Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: induction of ESM-1, (beta)ig-h3, and NrCAM PMID: 11866539
induction of Nr-CAM transcription plays a role in melanoma and colon cancer tumorigenesis PMID: 12183361
NrCAM is overexpressed in human papillary thyroid carcinoma at the mRNA and protein levels, whatever the tumour stage. PMID: 17667921
Distinct cytokeratin 7, cytokeratin 19, & neuronal cell adhesion molecule staining patterns are seen in hepatic adenoma & focal nodular hyperplasia possibly suggest activation of different subsets of hepatic progenitor/stem cell. PMID: 18602664
our results indicated that the NRCAM gene is one of the strong candidate genes for autism PMID: 18664314

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Secreted.

Protein Families

Immunoglobulin superfamily, L1/neurofascin/NgCAM family

Tissue Specificity

Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

Database Links

HGNC: 7994

OMIM: 601581

KEGG: hsa:4897

STRING: 9606.ENSP00000368314

UniGene: Hs.21422

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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