OPN1MW Antibody

Datasheet

Code	CSB-PA016352LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: OPN1MW antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa Immunofluorescence staining of HepG2 cells with CSB-PA016352LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) OPN1MW Polyclonal antibody

Uniprot No.

P04001

Target Names

OPN1MW

Alternative Names

OPN1MW; GCP; Medium-wave-sensitive opsin 1; Green cone photoreceptor pigment; Green-sensitive opsin; GOP

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Medium-wave-sensitive opsin 1 protein (1-52AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The OPN1MW Antibody (Product code: CSB-PA016352LA01HU) is Non-conjugated. For OPN1MW Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA016352LB01HU	OPN1MW Antibody, HRP conjugated	ELISA
FITC	CSB-PA016352LC01HU	OPN1MW Antibody, FITC conjugated
Biotin	CSB-PA016352LD01HU	OPN1MW Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Polyclonal rabbit anti-OPN1MW antibody raised against a recombinant protein containing the 1-52 amino acids of the human OPN1MW is recommended to detect the human OPN1MW protein. It was subjected to protein G purification and reached up to 95% in purity. Three applications including ELISA, WB, and IF have been used in assays to test for the specificity of this OPN1MW antibody.

OPN1MW is primarily expressed in the cone cells of the retina, where it is responsible for color vision and visual acuity in bright light conditions. Mutations in the OPN1MW gene have been associated with various visual disorders, including color blindness and macular degeneration.

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Target Background

Function

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.

Involvement in disease

Colorblindness, partial, deutan series (CBD); Blue cone monochromacy (BCM); Cone dystrophy 5 (COD5)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family, Opsin subfamily

Tissue Specificity

The three color pigments are found in the cone photoreceptor cells.

Database Links

HGNC: 4206

OMIM: 300821

KEGG: hsa:101060233

UniGene: Hs.247787

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

G protein coupled receptor signaling pathway

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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