OTOP1 Antibody

Datasheet

Code	CSB-PA752530LA01HU
Size	US$166
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Image	IHC image of CSB-PA752530LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA752530LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) OTOP1 Polyclonal antibody

Uniprot No.

Q7RTM1

Target Names

OTOP1

Alternative Names

OTOP1 antibody; Proton channel OTOP1 antibody; Otopetrin-1 antibody; hOtop1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Proton channel OTOP1 protein (414-544AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The OTOP1 Antibody (Product code: CSB-PA752530LA01HU) is Non-conjugated. For OTOP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA752530LB01HU	OTOP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA752530LC01HU	OTOP1 Antibody, FITC conjugated
Biotin	CSB-PA752530LD01HU	OTOP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Proton-selective channel that specifically transports protons into cells. Proton channel activity is only weakly-sensitive to voltage. Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes. In the vestibular system of the inner ear, required for the formation and function of otoconia, which are calcium carbonate crystals that sense gravity and acceleration. Probably acts by maintaining the pH appropriate for formation of otoconia. Regulates purinergic control of intracellular calcium in vestibular supporting cells. May be involved in sour taste perception in sour taste cells by mediating entry of protons within the cytosol. Also involved in energy metabolism, by reducing adipose tissue inflammation and protecting from obesity-induced metabolic dysfunction.

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Otopetrin family

Database Links

HGNC: 19656

OMIM: 607806

KEGG: hsa:133060

STRING: 9606.ENSP00000296358

UniGene: Hs.534544

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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