OTUD3 Antibody

Datasheet

Citations (1)

Code	CSB-PA719399LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: U251 whole cell lysate, U87 whole cell lysate All lanes: OTUD3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa Immunofluorescence staining of U251 cells with CSB-PA719399LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) OTUD3 Polyclonal antibody

Uniprot No.

Q5T2D3

Target Names

OTUD3

Alternative Names

KIAA0459 antibody; OTU domain-containing protein 3 antibody; OTUD3 antibody; OTUD3_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human OTU domain-containing protein 3 protein (108-398AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The OTUD3 Antibody (Product code: CSB-PA719399LA01HU) is Non-conjugated. For OTUD3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA719399LB01HU	OTUD3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA719399LC01HU	OTUD3 Antibody, FITC conjugated
Biotin	CSB-PA719399LD01HU	OTUD3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Rolapitant treats lung cancer by targeting deubiquitinase OTUD3 T Du,Cell communication and signaling,2024

Customer Reviews and Q&A

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Target Background

Function

Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains.

Gene References into Functions

OTUD3 is an essential regulator of PTEN and the OTUD3-PTEN signalling axis plays a critical role in tumour suppression. PMID: 26280536
Single-nucleotide polymorphism in OTUD3 gene is associated with ulcerative colitis. PMID: 23511034

Database Links

HGNC: 29038

OMIM: 611758

KEGG: hsa:23252

STRING: 9606.ENSP00000364261

UniGene: Hs.374987

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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