P4HA1 Antibody

Code CSB-PA017339LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Rat kidney tissue
    All lanes: P4HA1 antibody at 2.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 62, 61, 59 kDa
    Observed band size: 62 kDa

  • Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA017339LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA017339LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) P4HA1 Polyclonal antibody
Uniprot No.
Target Names
P4HA1
Alternative Names
2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody; 4-hydroxylase, alpha I subunit antibody; 4-PH alpha-1 antibody; P4HA antibody; P4HA1 antibody; P4HA1_HUMAN antibody; Procollagen-proline antibody; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I antibody; Prolyl 4-hydroxylase subunit alpha-1 antibody
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Prolyl 4-hydroxylase subunit alpha-1 protein (256-525AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The P4HA1 Antibody (Product code: CSB-PA017339LA01HU) is Non-conjugated. For P4HA1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA017339LB01HU P4HA1 Antibody, HRP conjugated ELISA
FITC CSB-PA017339LC01HU P4HA1 Antibody, FITC conjugated
Biotin CSB-PA017339LD01HU P4HA1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Gene References into Functions
  1. miR-122 inhibited migration, invasion, epithelial mesenchymal transition, and metastasis in peritoneal cavity of ovarian cancer cells by targeting P4HA1. PMID: 30136751
  2. High expression of P4HA1 was correlated with the malignancy of gliomas and could serve as a prognostic indicator for patients with high-grade gliomas PMID: 28964577
  3. Our study indicates that P4HA1 plays a pivotal role in the process of GSC-EC transdifferentiation and the structural formation of vascular BMs. PMID: 28415787
  4. we report compound heterozygous frameshift and splice site mutations in P4HA1 that impair but not abolish C-P4H alpha(I) activity. The maternal P4HA1 exon 12 splice donor site mutation causes an internally deleted C-P4H alpha(I) predicted to completely lack catalytic activity. two nucleic acid insertion in exon 9 results in a premature stop in the exon 9 P4HA1 splice form. PMID: 28419360
  5. Findings suggest that the catalytic domain of collagen prolyl 4-hydroxylases (CP4Hs) recognizes the cis conformation of the prolyl peptide bond. PMID: 28001367
  6. Thus, we conclude that miR-30e suppresses proliferation of hepatoma cells through targeting P4HA1 mRNA. PMID: 26966067
  7. Studies indicate P4HA1 copy number gain in a subset of metastatic prostate tumors and its expression is also regulated by microRNA-124. PMID: 25115393
  8. Overexpression of miR-122 markedly attenuated the expression of P4HA1 via targeting a binding site located at 3'-UTR of P4HA1 mRNA PMID: 23178710
  9. Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts. PMID: 23423382
  10. IL-6 significantly downregulated P4Halpha1 expression in aortic smooth muscle cells PMID: 23022409
  11. analysis of the 2-His-1-Asp active-site motif in prolyl 4-hydroxylase PMID: 19890397
  12. These in vivo data demonstrated that smokers had thinner atherosclerotic cap thickness and lower levels of P4Halpha and collagen. PMID: 15369792
  13. Collagen prolyl 4-hydroxylase-alpha (I)mRNA is stabilized by interation of RNA-binding proteins hnRNP-A2/B1 with a U(16) element within the 3'-UTR PMID: 16464861
  14. positive (transforming growth factor beta1) and negative (cigarette smoking extract) regulators appear to influence the USF-E-box interaction and affect P4Halpha(I) expression PMID: 16488890
  15. Results suggest that the alteration of translational efficiency by nucleolin, which occurs through a hypoxia inducible factor independent pathway, is an important step in collagen prolyl 4-hydroxylase-alpha(I) regulation under hypoxia. PMID: 16837461
  16. In comparison with healthy cartilage, Osteoarthritis articular chondrocytes exhibit increased in vivo synthesis of collagen prolyl-4-hydroxylase type II, a pivotal enzyme in collagen triple helix formation. PMID: 16877351
  17. HIF-P4H, HIF-1alpha and HIF-2alpha are effective oxygen sensors PMID: 16885164

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Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
P4HA family
Database Links

HGNC: 8546

OMIM: 176710

KEGG: hsa:5033

STRING: 9606.ENSP00000263556

UniGene: Hs.500047

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