PBK Antibody

Code CSB-PA822241DSR1HU
Size US$166
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  • Western blot
    All lanes: PBK antibody at 6μg/ml
    Lane 1: Hela whole cell lysate
    Lane 2: Mouse gonad tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 37, 38 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA822241DSR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA822241DSR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human thymus tissue using CSB-PA822241DSR1HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PBK Polyclonal antibody
Uniprot No.
Target Names
PBK
Alternative Names
Cancer/testis antigen 84 antibody; CT84 antibody; Epididymis luminal protein 164 antibody; FLJ14385 antibody; HEL164 antibody; Lymphokine activated killer T cell originated protein kinase antibody; Lymphokine-activated killer T-cell-originated protein kinase antibody; MAPKK like protein kinase antibody; MAPKK-like protein kinase antibody; Nori 3 antibody; Nori-3 antibody; Nori3 antibody; PBK antibody; PDZ binding kinase antibody; PDZ-binding kinase antibody; Serine/threonine protein kinase antibody; Spermatogenesis related protein kinase antibody; Spermatogenesis-related protein kinase antibody; SPK antibody; T LAK cell originated protein kinase antibody; T-LAK cell-originated protein kinase antibody; TOPK antibody; TOPK_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Lymphokine-activated killer T-cell-originated protein kinase protein (1-322AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:1000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Gene References into Functions
  1. Results found that PBK/TOPK was upregulated and phosphorylated in HTLV1-transformed Tcell lines and ATLLderived Tcell lines. In addition, HTLV1 infection upregulated PBK/TOPK expression in PBMCs, suggesting that this kinase increases the rate of mitosis and expands malignant T cells. PMID: 29901068
  2. Findings strongly suggest that phosphorylated T-lymphokine-activated killer cell-originated protein kinase may be a potent biomarker to determine the outcome of patients with primary central nervous system lymphoma and to develop novel drugs to treat primary central nervous system lymphoma. PMID: 29575092
  3. the expression of TOPK be a novel alternative biomarker to categorize the malignancy of gliomas. PMID: 29271010
  4. CDK1-mediated mitotic phosphorylation of PDZ-binding kinase is involved in cytokinesis and inhibits its oncogenic activity. PMID: 28780319
  5. Data suggest that T-LAK cell-originated protein kinase (TOPK) might play a pivotal role in breast cancer invasion and metastasis. PMID: 28212583
  6. our study provide convincing evidences that FoxM1-regulated PBK exerts oncogenic activities towards HCC via the activation of beta-Catenin pathway. PMID: 28525379
  7. High TOPK expression is associated with liver cancer. PMID: 27582549
  8. The role of T-LAK cell-originated protein kinase directly promotes metastasis of colorectal cancer by modulating p53-related protein kinase. PMID: 28412249
  9. TOPK protein and transcriptional levels of FOXM1 were reduced by TOPK inhibitor treatment. PMID: 27334838
  10. Results indicate that src-family kinase (Src) is a upstream kinase of T-LAK cell-originated protein kinase (TOPK). PMID: 27016416
  11. Upregulation of proteasome subunit beta type 8 PSMB8 and PDZ binding kinase PBK was confirmed by real-time reverse transcription-PCR analysis. PMID: 26894977
  12. MiR-770-5p and PBK levels were inversely correlated in xenograft model mice. PMID: 28333152
  13. TOPK knockdown did not alter radiation response in normal tissues, but significantly enhanced radiosensitivity in cancer cells.These findings have clinical relevance, as elevated TOPK protein expression was associated with poorer clinical outcomes in prostate cancer patients treated with radical radiotherapy. TOPK disruption may cause tumour-specific radiosensitisation in multiple different tumour types. PMID: 28677687
  14. Determined the crystal structure of PBK, which has two phospho-mimicking mutations T9E and T198E. The structural data indicated that PBK may assemble into an inactive dimer in alkaline conditions. Analytical size-exclusion chromatography and analytical ultracentrifugation confirmed that PBK exists in a conformational transition between dimers and monomers at different pH conditions. PMID: 27262437
  15. PBK/TOPK plays a crucial role in tumour malignant potential through its overexpression and highlight its usefulness as a prognostic factor and potential therapeutic target in gastric cancer. PMID: 27898655
  16. A high PBK expression level was correlated with long overall survival in oral squamous cell carcinoma. PMID: 27347940
  17. findings suggest that PBK/TOPK plays a crucial role in tumor malignant potential through its overexpression in ESCC PMID: 27919968
  18. TOPK silencing sensitizes EGFR-TKI-resistant lung cancer cells to gefitinib and increases gefitinib efficacy in preclinical lung adenocarcinoma xenograft models. PMID: 26745678
  19. Study shows that BUB1beta and PDZ binding kinase are up-regulated in breast cancer tumors and their expression is associated with improved overall survival (OS) in basal-like tumors but worse OS in luminal and untreated patients. PMID: 26897635
  20. Overexpression of Nrf2 inhibited the PBK/TOPK KD-induced decrease in cdc2 and cyclin B expression and cell cycle arrest, and blocked ROS production and apoptosis. PMID: 26503118
  21. The in vitro data have been consistent with a role for PBK/TOPK in facilitating invasion in prostate cancer. PBK could be a prognostic biomarker for prostate cancer that would discriminate aggressive prostate cancer from indolent disease. PMID: 25909225
  22. TOPK was speculated to be one of a potential marker and therapeutic target in advanced prostate cancer PMID: 25881543
  23. Authors identified TOPK/PBK, in vitro and in vivo, as the master ZFP linker kinase. Furthermore, they show precise temporal correlation between TOPK activating phosphorylation by Cdk1 and linker phosphorylation in mitosis. PMID: 25575812
  24. PBK/TOPK expression is closely associated with cervical cancer and cervical intraepithelial neoplasia, which may be served as a useful target for tumor diagnosis and immunotherapy. PMID: 25550851
  25. TOPK, overexpressed in colorectal cancer, enhances the resistance of colorectal cancer cells to anoikis. PMID: 25687885
  26. PDZ-binding kinase/T-LAK cell-originated protein kinase correlates with mutant p53 and affects cell proliferation and viability as well as prognosis in lung adenocarcinoma PMID: 25466965
  27. Study findings suggest that the PBK/TOPK mRNA/protein expression is specific to human bladder cancer and might be used as a novel target for development of cancer immunotherapy and diagnostic biomarker. PMID: 24629784
  28. TOPK protein upregulates iNOS gene expression in T cell leukemia Jurkat cells or macrophage leukemic Raw 264.7 cells via NF-kappaB activation in response to LPS, and might act as a critical effector in LPS/TLR4-mediated signaling cascade. PMID: 24440499
  29. PBK/TOPK expression is positively correlated with Ki67 and p53 expression, and can be used as an independent prognostic factor in non-small-cell lung cancer. PMID: 24025073
  30. These results suggest that the malignant transformation of plexiform neurofibroma is associated with distinct changes in the expression of BUB1B, PBK and NEK2 PMID: 23370767
  31. The 3D structure of TOPK protein has been constructed by using multiple templates. PMID: 22940854
  32. that the serine-threonine kinase PBK/TOPK is frequently overexpressed in high-grade lymphomas and its expression is positively correlated to that of c-Myc and E2F1 PMID: 23237560
  33. TOPK directly interacted with and phosphorylated IkappaBalpha at Ser-32, leading to p65 nuclear translocation and NF-kappaB activation. PMID: 23250755
  34. Our results indicate that overexpression of TOPK could predetermine the metastatic capability of tumors and could serve as a significant prognostic predictor of shortened overall survival and time to recurrence. PMID: 22192142
  35. T-LAK cell-originated protein kinase (TOPK) phosphorylation of MKP1 protein prevents solar ultraviolet light-induced inflammation through inhibition of the p38 protein signaling pathway. PMID: 21715333
  36. Upregulation of TOPK is associated with breast cancer. PMID: 20589935
  37. increased levels of PBK/TOPK may contribute to tumor cell development and progression through suppression of p53 function and consequent reductions in the cell-cycle regulatory proteins such as p21. PMID: 20622899
  38. Phosphorylation of Prx1 (Ser-32) by TOPK prevents UVB-induced apoptosis in RPMI7951 melanoma cells through regulation of Prx1 peroxidase activity and blockade of intracellular H(2)O(2) accumulation. PMID: 20647304
  39. TOPK is indispensable for cancer cell cytokinesis throughout phosphorylation on p97. PMID: 19900192
  40. PBK/TOPK protein could serve as a useful indicator for histopathologic differentiation between cholangiocarcinoma and hepatocellular carcinomas and the low expression of PBK/TOPK is predicative of poor survival in cholangiocarcinoma patients. PMID: 19954816
  41. PBK/TOPK is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells and during normal fetal development. PMID: 11783945
  42. TOPK protein is up-regulated in a variety of hematologic malignancies and may be involved in leukemic cell growth; 4 of 5 clinical that strongly expressed TOPK also strongly expressed phosphorylated c-Myc PMID: 14757441
  43. PBK/TOPK plays an important role in transiently amplifying neural progenitors in the adult subependymal zone of transgenically targeted mice. PMID: 16291951
  44. PBK augments tumor cell growth following transient appearance in different types of progenitor cells in vivo as reported. PMID: 17482142
  45. Findings showed that TOPK positively modulated UVB-induced JNK1 activity and played a pivotal role in JNK1-mediated cell transformation induced by H-Ras. PMID: 17545598
  46. The positive feedback loop between TOPK and ERK2 increases tumorigenesis properties of HCT116 colorectal cancer cells, and TOPK-regulated signaling may serve as a potential therapeutic target in colorectal cancer PMID: 17631144

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Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Database Links

HGNC: 18282

OMIM: 611210

KEGG: hsa:55872

STRING: 9606.ENSP00000301905

UniGene: Hs.104741

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