PCOLCE2 Antibody

Datasheet

Citations (2)

Code	CSB-PA017632LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A431 whole cell lysate(30µg), A549 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), 293 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), U87 whole cell lysate(30µg),A2780 whole cell lysate(30µg), Mouse Kidney tissue lysate(30µg) All lanes: PCOLCE2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 120s Immunofluorescence staining of A549 cell with CSB-PA017632LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of MCF-7 cell with CSB-PA017632LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of MCF-7cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PCOLCE2 Polyclonal antibody

Uniprot No.

Q9UKZ9

Target Names

PCOLCE2

Alternative Names

2400001O18Rik antibody; MGC187469 antibody; PCOC2_HUMAN antibody; PCOLCE 2 antibody; Pcolce2 antibody; PCOLE 2 antibody; PCOLE2 antibody; PCPE 2 antibody; PCPE-2 antibody; PCPE2 antibody; Procollagen C endopeptidase enhancer 2 antibody; Procollagen C proteinase enhancer 2 antibody; Procollagen C-endopeptidase enhancer 2 antibody; Procollagen C-proteinase enhancer 2 antibody; Procollagen COOH terminal proteinase enhancer 2 antibody; Procollagen COOH-terminal proteinase enhancer 2 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Procollagen C-endopeptidase enhancer 2 protein (24-415AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PCOLCE2 Antibody (Product code: CSB-PA017632LA01HU) is Non-conjugated. For PCOLCE2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017632LB01HU	PCOLCE2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA017632LC01HU	PCOLCE2 Antibody, FITC conjugated
Biotin	CSB-PA017632LD01HU	PCOLCE2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Identification of extracellular matrix-related biomarkers in colon adenocarcinoma by bioinformatics and experimental validation Y Yin,Frontiers in immunology,2024
Pcolce2 overexpression promotes supporting cell reprogramming in the neonatal mouse cochlea C Xu,Cell proliferation,2024

Customer Reviews and Q&A

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Target Background

Function

Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.

Gene References into Functions

Procollagen C-endopeptidase protein 2, atherosclerosis and HDL cholesteryl ester catabolism. [Review] PMID: 26218419
Assignment of Pcolce2 gene, which encodes procollagen C-proteinase enhancer protein 2, to human chromosome 3q23. PMID: 12063410
PCPE2 is shown to be a glycoprotein that differs markedly in the nature of its glycosylation from that of PCPE1 PMID: 12393877
Data indicate that PCPE2 accelerates the proteolytic processing of pro-apolipoprotein (apo) AI by enhancing the cleavage of the hexapeptide extension present at the N terminus of apoAI. PMID: 19237735

Subcellular Location

Secreted.

Tissue Specificity

Highly expressed in the heart, trabecular meshwork, pituitary gland, bladder, mammary gland, trachea and placenta and weakly expressed in the brain. Expressed in cartilage.

Database Links

HGNC: 8739

OMIM: 607064

KEGG: hsa:26577

STRING: 9606.ENSP00000295992

UniGene: Hs.8944

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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