PFKL Antibody

Code CSB-PA017821LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, HL60 whole cell lysate, Mouse liver tissue
    All lanes: PFKL antibody at 2.8μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 86, 91 kDa
    Observed band size: 86, 50, 40 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA017821LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA017821LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PFKL Polyclonal antibody
Uniprot No.
Target Names
PFKL
Alternative Names
PFKL antibody; ATP-dependent 6-phosphofructokinase antibody; liver type antibody; ATP-PFK antibody; PFK-L antibody; EC 2.7.1.11 antibody; 6-phosphofructokinase type B antibody; Phosphofructo-1-kinase isozyme B antibody; PFK-B antibody; Phosphohexokinase antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human ATP-dependent 6-phosphofructokinase, liver type protein (229-479AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PFKL Antibody (Product code: CSB-PA017821LA01HU) is Non-conjugated. For PFKL Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA017821LB01HU PFKL Antibody, HRP conjugated ELISA
FITC CSB-PA017821LC01HU PFKL Antibody, FITC conjugated
Biotin CSB-PA017821LD01HU PFKL Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Applications : WB

Sample type: cell

Review: PFK, PKM, and LDHA in the liver of the embryos in ovo injected with 20 mg/kg 2-DG or 10 nM DAPT in the yolk sac (n = 6).

By Anonymous

Target Background

Function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway.
Gene References into Functions
  1. Liver PFK1 isoform assembles into filaments in a tetramer- and substrate-dependent manner,organizing isoform specific glucose metabolism in cells. PMID: 28646105
  2. findings showed glycosylation inhibited PFK1 activity and redirected glucose flux through the pentose phosphate pathway, thereby conferring a selective growth advantage on cancer cells PMID: 22923583
Subcellular Location
Cytoplasm.
Protein Families
Phosphofructokinase type A (PFKA) family, ATP-dependent PFK group I subfamily, Eukaryotic two domain clade "E" sub-subfamily
Database Links

HGNC: 8876

OMIM: 171860

KEGG: hsa:5211

STRING: 9606.ENSP00000269848

UniGene: Hs.255093

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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