PIK3IP1 Antibody

Datasheet

Code	CSB-PA850293LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue All lanes: PIK3IP1 antibody at 7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 26, 12, 19, 20 kDa Observed band size: 29 kDa Immunofluorescence staining of Hela cells with CSB-PA850293LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PIK3IP1 Polyclonal antibody

Uniprot No.

Q96FE7

Target Names

PIK3IP1

Alternative Names

HGFL antibody; hHGFL(S) antibody; Kringle domain containing protein HGFL antibody; Kringle domain-containing protein HGFL antibody; MGC17330 antibody; P3IP1_HUMAN antibody; Phosphoinositide 3 kinase interacting protein 1 antibody; Phosphoinositide-3-kinase-interacting protein 1 antibody; PIK3IP1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Phosphoinositide-3-kinase-interacting protein 1 protein (22-168AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PIK3IP1 Antibody (Product code: CSB-PA850293LA01HU) is Non-conjugated. For PIK3IP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA850293LB01HU	PIK3IP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA850293LC01HU	PIK3IP1 Antibody, FITC conjugated
Biotin	CSB-PA850293LD01HU	PIK3IP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Negative regulator of hepatic phosphatidylinositol 3-kinase (PI3K) activity.

Gene References into Functions

Findings indicate that colon cancer associated transcript 1 (non-protein coding) (CCAT1) was upregulated in osteosarcoma tissues and cells, and was involved in the proliferation and migration of osteosarcoma via regulating miR-148a/phosphatidyl inositol 3-kinase interacting protein 1 (PIK3IP1) signal pathway. PMID: 28549102
These results suggest that the novel PI3K regulator PIK3IP1 plays an inhibitory role in T-cell activation. PMID: 22706993
PIK3IP1-v1 is a novel splicing isoform of PIK3IP1. Both of them are located on cell membrane and can induce cell apoptosis. PMID: 19088825
PIK3IP1 directly binds to the p110 catalytic subunit and down modulates PI3K activity. Suggested that PIK3IP1 is a new type of PI3K regulator. PMID: 17475214
PIK3IP1 is an important regulator of PI3K in vivo, and its dysregulation can contribute to liver carcinogenesis PMID: 18632611

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Database Links

HGNC: 24942

KEGG: hsa:113791

STRING: 9606.ENSP00000215912

UniGene: Hs.26670

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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