PIP4K2B Antibody

Datasheet

Code	CSB-PA018023ESR2HU
Size	US$166
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Image	Western blot All lanes: Phosphatidylinositol 5-phosphate 4-kinase type-2 beta antibody at 10μg/ml Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 33 kDa Observed band size: 48 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA018023ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018023ESR2HU at dilution of 1:100 Immunofluorescent analysis of A549 cells using CSB-PA018023ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PIP4K2B Polyclonal antibody

Uniprot No.

P78356

Target Names

PIP4K2B

Alternative Names

PIP4K2B antibody; PIP5K2B antibody; Phosphatidylinositol 5-phosphate 4-kinase type-2 beta antibody; EC 2.7.1.149 antibody; 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta antibody; Diphosphoinositide kinase 2-beta antibody; Phosphatidylinositol 5-phosphate 4-kinase type II beta antibody; PI(5)P 4-kinase type II beta antibody; PIP4KII-beta antibody; PtdIns(5)P-4-kinase isoform 2-beta antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Phosphatidylinositol 5-phosphate 4-kinase type-2 beta protein (1-270AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration. Its GTP-sensing activity is critical for metabolic adaptation. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3.

Gene References into Functions

PIP4K2B couples the cellular GTP concentration to the levels of the stress-regulated phosphoinositide PtdIns5P, to impact on tumor cell growth. PMID: 27132569
Suppression of PIP4K expression selectively prevents tumor cell growth in vitro and prevents tumor development in mice that have lost the tumor suppressor p53. (Review) PMID: 25728392
Association between low PIP4K2B expression and poor patient survival.Analysis of gene expression datasets confirmed the association between low PIP4K2B and low CDH1expression. PMID: 24127122
Knocking down PI5P4Kalpha and beta in a breast cancer cell line bearing an amplification of the gene encoding PI5P4K beta and deficient for p53 impaired growth on plastic and in xenografts. PMID: 24209622
PIPkin IIbeta is expressed as a tagged protein, is active as revealed by immunoprecipitation and enzyme assay, and that cellular fractionation reveals that it is indeed nuclear. PMID: 17303380

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Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm.

Tissue Specificity

Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.

Database Links

HGNC: 8998

OMIM: 603261

KEGG: hsa:8396

STRING: 9606.ENSP00000269554

UniGene: Hs.260603

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Regulation of actin cytoskeleton Phosphatidylinositol signaling system

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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