PKN3 Antibody

Code CSB-PA744059LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate
    All lanes: PKN3 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 100 kDa
    Observed band size: 100 kDa

  • IHC image of CSB-PA744059LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA744059LA01HU diluted at 1:300 and staining in paraffin-embedded human stomach cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PKN3 Polyclonal antibody
Uniprot No. Q6P5Z2
Target Names PKN3
Alternative Names pkn3 antibody; PKN3_HUMAN antibody; PKNBETA antibody; Protein kinase C related kinase 3 antibody; Protein kinase N3 antibody; Protein kinase PKN beta antibody; Protein kinase PKN-beta antibody; Protein-kinase C-related kinase 3 antibody; RP11-545E17.1 antibody; Serine/threonine protein kinase N3 antibody; Serine/threonine-protein kinase N3 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Serine/threonine-protein kinase N3 protein (1-252AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Contributes to invasiveness in malignant prostate cancer.
Gene References into Functions
  1. Steady-state kinetic analysis revealed that PKN1-3 follows a sequential ordered Bi-Bi kinetic mechanism, where peptide substrate binding is preceded by ATP binding. This kinetic mechanism was confirmed by additional kinetic studies for product inhibition and affinity of small molecule inhibitors. PMID: 27919031
  2. PKN3 is the major regulator of angiogenesis in humans and mice and tumor metastasis in mice. PMID: 26742562
  3. PKN3 can be considered a novel protein implicated in remodeling the actin-adherens junction, possibly by linking ICAM-1-signaling with actin/AJ dynamics. PMID: 22609186
  4. PKN isoforms are not simply redundant in supporting migration, but appear to be linked through isoform specific regulatory domain properties to selective upstream signals. It PMID: 21754995
  5. PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of phosphoinositide 3-kinase PMID: 15282551

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Subcellular Location Nucleus, Cytoplasm, perinuclear region
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
Tissue Specificity Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.
Database Links

HGNC: 17999

OMIM: 610714

KEGG: hsa:29941

STRING: 9606.ENSP00000291906

UniGene: Hs.300485

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