Code | CSB-PA744059LA01HU |
Size | US$166 |
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The PKN3 Antibody (Product code: CSB-PA744059LA01HU) is Non-conjugated. For PKN3 Antibody with conjugates, please check the following table.
Application | Recommended Dilution |
---|---|
WB | 1:1000-1:5000 |
IHC | 1:200-1:500 |
Applications : Western Blot (WB)
Sample type: HEK293T - human cell line
Sample dilution: 1:3000
Review: Native total lysates were submitted to co-IP using GFP agarose. PKN3 was detected as a co-immunoprecipitated protein (lanes 2-4, upper panel) and as total lysate (lanes 1-4, lower panel). The antibody was diluted in 1% BSA in TBST (1:3000), secondary Ab in 2% milk in TBST. The antibody is good for detection of co-immunoprecipitated PKN3, but not for PKN3 in the whole cell lysate samples.
By Anonymous