PKN3 Antibody

Datasheet

Code	CSB-PA744059LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate All lanes: PKN3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 100 kDa Observed band size: 100 kDa IHC image of CSB-PA744059LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA744059LA01HU diluted at 1:300 and staining in paraffin-embedded human stomach cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PKN3 Polyclonal antibody

Uniprot No.

Q6P5Z2

Target Names

PKN3

Alternative Names

pkn3 antibody; PKN3_HUMAN antibody; PKNBETA antibody; Protein kinase C related kinase 3 antibody; Protein kinase N3 antibody; Protein kinase PKN beta antibody; Protein kinase PKN-beta antibody; Protein-kinase C-related kinase 3 antibody; RP11-545E17.1 antibody; Serine/threonine protein kinase N3 antibody; Serine/threonine-protein kinase N3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine/threonine-protein kinase N3 protein (1-252AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PKN3 Antibody (Product code: CSB-PA744059LA01HU) is Non-conjugated. For PKN3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA744059LB01HU	PKN3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA744059LC01HU	PKN3 Antibody, FITC conjugated
Biotin	CSB-PA744059LD01HU	PKN3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

3.0 - 1 reviews

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Applications : Western Blot (WB)

Sample type: HEK293T - human cell line

Sample dilution: 1:3000

Review: Native total lysates were submitted to co-IP using GFP agarose. PKN3 was detected as a co-immunoprecipitated protein (lanes 2-4, upper panel) and as total lysate (lanes 1-4, lower panel). The antibody was diluted in 1% BSA in TBST (1:3000), secondary Ab in 2% milk in TBST. The antibody is good for detection of co-immunoprecipitated PKN3, but not for PKN3 in the whole cell lysate samples.

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Target Background

Function

Contributes to invasiveness in malignant prostate cancer.

Gene References into Functions

Steady-state kinetic analysis revealed that PKN1-3 follows a sequential ordered Bi-Bi kinetic mechanism, where peptide substrate binding is preceded by ATP binding. This kinetic mechanism was confirmed by additional kinetic studies for product inhibition and affinity of small molecule inhibitors. PMID: 27919031
PKN3 is the major regulator of angiogenesis in humans and mice and tumor metastasis in mice. PMID: 26742562
PKN3 can be considered a novel protein implicated in remodeling the actin-adherens junction, possibly by linking ICAM-1-signaling with actin/AJ dynamics. PMID: 22609186
PKN isoforms are not simply redundant in supporting migration, but appear to be linked through isoform specific regulatory domain properties to selective upstream signals. It PMID: 21754995
PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of phosphoinositide 3-kinase PMID: 15282551

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Subcellular Location

Nucleus. Cytoplasm, perinuclear region. Note=Nuclear and perinuclear Golgi region.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Tissue Specificity

Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.

Database Links

HGNC: 17999

OMIM: 610714

KEGG: hsa:29941

STRING: 9606.ENSP00000291906

UniGene: Hs.300485

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PI3K-Akt signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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