PKN3 Antibody

Datasheet
Code CSB-PA744059LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q6P5Z2
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate
    All lanes: PKN3 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 100 kDa
    Observed band size: 100 kDa

  • IHC image of CSB-PA744059LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA744059LA01HU diluted at 1:300 and staining in paraffin-embedded human stomach cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Serine/threonine-protein kinase N3 protein (1-252AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500
Relevance Contributes to invasiveness in malignant prostate cancer.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Serine/threonine-protein kinase N3 (EC 2.7.11.13) (Protein kinase PKN-beta) (Protein-kinase C-related kinase 3), PKN3, PKNBETA
Immunogen Species Homo sapiens (Human)
Research Area Cell Biology
Target Names PKN3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Contributes to invasiveness in malignant prostate cancer.
Subcellular Location Nucleus, Cytoplasm, perinuclear region
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
Tissue Specificity Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.
Database Links

HGNC: 17999

OMIM: 610714

KEGG: hsa:29941

STRING: 9606.ENSP00000291906

UniGene: Hs.300485

Pathway PI3K-Akt signaling pathway

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