PLIN2 Antibody

Code CSB-PA920084
Size US$299
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA920084(PLIN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA920084(PLIN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue, Primary antibody: CSB-PA920084(PLIN2 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
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Product Details

Uniprot No. Q99541
Target Names PLIN2
Alternative Names ADFP antibody; Adipophilin antibody; Adipose differentiation related protein antibody; Adipose differentiation-related protein antibody; ADRP antibody; MGC10598 antibody; Perilipin-2 antibody; PLIN2 antibody; PLIN2_HUMAN antibody
Raised in Rabbit
Species Reactivity Human,Mouse
Immunogen Synthetic peptide of Human PLIN2
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method Antigen affinity purification
Concentration It differs from different batches. Please contact us to confirm it.
Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form Liquid
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
ELISA 1:2000-1:5000
WB 1:500-1:2000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Anti-steatotic linagliptin pleiotropic effects encompasses suppression of de novo lipogenesis and ER stress in high-fat-fed mice. F de Oliveira Santos,Molecular and Cellular Endocrinology,2020

Applications: Immunofluorescence
Antibody dilution factor: 1:50
Sample species: Mice
Review: The immunostaining for PLIN2 revealed the highest label was observed in the HF group (green label, white arrowheads), whereas the HF-L group presented with a significantly reduced label.
PMID: 32259637

The deficiency and the supplementation of vitamin D and liver: lessons of chronic fructose-rich diet in mice. Maia-Ceciliano TC,The Journal of Steroid Biochemistry and Molecular Biology,2019

Applications: IF
Antibody dilution factor: 1: 100
Review: Representative sections of the liver prepared for immunofluorescence with anti-Plin2 (green, confocal scanning laser microscopy to identify a constitutively associated cytoplasmic lipid droplet coat protein, same magnification in all photomicrographs). The identification of the groups is indicated in the left upper corner. There was increased Plin2 stain in CDD, and still more pronounced in F and FDD. In CDS and FDS the liver structure was restored.
PMID: 31175967

Intermittent fasting beneficial effects on the steatosis and inflammation of the liver in mice fed a high-fat or a high-fructose diet. Thatiany de SouzaMarinho, et al,Nutrition,2019

Applications: IF
Review: Confocal laser scanning microscopy (blue: cell nuclei, DAPI; green: PLIN 2, Alexa 488). Abbreviations: C (control), IF (intermittent fasting), HF (high-fat), HFr (high-fructose).

Empaglifozin mitigates NAFLD in high-fat-fed mice by alleviating insulin resistance, lipogenesis and ER stress. Petito-da-Silva TI, et al,Molecular and Cellular Endocrinology,2019

Applications: IF
Antibody dilution factor: 1: 100
Review: the immunohistochemical staining for Plin2 protein 819 was more labelling in HF group, therefore the HF-EMPA presented less labelling, in second row.
PMID: 31419466

Target Data

Function May be involved in development and maintenance of adipose tissue.
Gene References into Functions
  1. Study utilizing THP-1 macrophages concludes that long-chain metabolite of vitamin E, alpha-13'-carboxychromanol, protects cells from lipotoxicity, at least partially via PLIN2 regulation. PMID: 29733984
  2. PLIN2 protein levels are significantly increased in patients with neoatherosclerosis. PMID: 29422264
  3. hese results suggested that PLIN2 may be considered a novel prognostic factor in Clear cell renal cell carcinoma (ccRCC) and a specific diagnostic indicator for patients with ccRCC. Furthermore, it could be a potential novel target for the clinical treatment of ccRCC PMID: 29749470
  4. ADRP knockdown significantly inhibited PDGFinduced increases in Vascular smooth muscle cell viability, caused G1 phase cell cycle arrest and decreased PCNA expression. PMID: 28713961
  5. this papet shows that adipophilin is a novel prognostic biomarker in clear cell renal cell carcinoma PMID: 28404922
  6. PLIN2 gene Ser251Pro missense mutation is associated with reduced insulin secretion and increased insulin sensitivity. PMID: 26443937
  7. Interference with PLIN2 and PPARalpha resulted in major alterations in gene expression, especially affecting lipid, glucose, and purine metabolism. PMID: 27308945
  8. This study shows that hepatitis C virus suppresses hepatic ADRP expression in infected patients and cell lines. Forcing the expression of miR-148a and miR-30a limits the suppressive effect of hepatitis C virus on ADRP. PMID: 27591428
  9. ADP was expressed in a small proportion of lung adenocarcinoma suggesting that ADP-positive lung adenocarcinoma could be a distinct subtype of lung adenocarcinoma, induced by up-regulation of the lipogenic pathway. PMID: 27467545
  10. Activation of ARF1 dissociates ADRP from lipid droplets. A constitute active form of ARF1 (ARF1Q71I) promotes HCV assembly. ADRP played a positive role in Hepatitis C virus replication and negative role in Hepatitis C virus assembly. PMID: 27157138
  11. PLIN2 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  12. Adipophilin was present in 24 of 26 colorectal hyperplastic polyps, but did not correlate with presence of white opaque substance (i.e. lipid droplets) on magnifying endoscopy with narrow band imaging. PMID: 26636961
  13. Conserved amphipathic helices mediate lipid droplet targeting of PLIN1, PLIN2, and PLIN3. PMID: 26742848
  14. In hepatitis C virus -infected human livers, occludin and ADRP mRNA expression levels correlated with each other. Hepatitis C virus increases occludin expression via the upregulation of ADRP. PMID: 26731658
  15. PLIN2 involves lipid modulation of GSK3 activity, GSK3 substrate expression, and cell growth/survival. PMID: 26598603
  16. PLIN2 inhibits insulininduced glucose uptake by activating NLRP3, caspase1 and IL1beta, leading to a decreased IRS1 expression. PMID: 26166692
  17. Plin2 appears to be associated with functional alterations of the muscle PMID: 25559404
  18. Results demonstrate the clinical utility, specificity, and sensitivity of urine AQP1 and PLIN2 to diagnose renal cell carcinoma. PMID: 26181025
  19. Postprandial triglyceride rich lipoproteins may be involved in atherosclerotic plaque formation through the regulation of perilipin-2 and perilipin-3 proteins in macrophages. PMID: 25595097
  20. Immunohistochemical distinction of renal cell carcinoma from other carcinomas with clear-cell histomorphology: utility of CD10 and CA-125 in addition to PAX-2, PAX-8, RCCma, and adipophilin. PMID: 25279712
  21. ADRP was detectable in human BM during the whole 12 months of lactation period and its levels were intraindividually well-conserved. PMID: 24577896
  22. Perilipin 2 binding to the lipid droplet surface requires both hydrophobic and electrostatic interactions. PMID: 25172666
  23. Fat deposition within skeletal muscle in form of Plin2-coated lipid droplets increases with age and is associated with decreased muscle strength and thickness, likely through an IGF-1- and p53-dependent mechanism. PMID: 23977392
  24. Mammary analogue secretory carcinoma is a lipid-rich tumour containing large lipid droplets covered by adipophilin. PMID: 23931576
  25. Overexpression of ADRP alone increased the formation of foam cells and consequently autophagic activity. PMID: 24405744
  26. Data indicate that the allele of the missense polymorphism Ser251Pro disrupts a predicted alpha-helix in perilipin 2 (PLIN2). PMID: 23603836
  27. PLIN2 is the major perilipin regulated during sebocyte differentiation in vitro. PLIN2 is also important for sebaceous lipid accumulation in vitro and regulates sebaceous gland size in vivo PMID: 23688400
  28. Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5 PMID: 23129790
  29. Gene expression profile indicated a significant over-expression of the adipophilin gene and marked up-regulation of other genes involved in lipid metabolism in Burkitt lymphoma. PMID: 22952953
  30. Data suggest that prolonged endurance exercise training increases expression of perilipin 2 alongside increases in intramuscular triglyceride content specificly in type I/slow-twitch muscle fibers of type 2 diabetes patients. PMID: 22949030
  31. It was shown that the PLIN2 content in skeletal muscle is unchanged in response to a single bout of endurance exercise. The PLIN2-intramuscular triglyceride association was reduced postexercise. PMID: 22496505
  32. PLIN2-PLIN5 proteins were all more abundant in women than in men (p = 0.037 and p < 0.0001, respectively), consistent with higher intramyocellular lipid content observed in female skeletal muscle. PMID: 22667335
  33. High PLIN2 protein level is associated with colorectal cancer. PMID: 21828233
  34. The frequency of adipophilin-positive cases was significantly higher in apocrine breast carcinomas compared with nonapocrine carcinomas. PMID: 21566511
  35. Adipophilin-positive macrophage infiltration in the fibrous cap might be correlated with instability in neurological status PMID: 20887090
  36. Perilipin may play a role in the long-term storage of fat, which may be only partially reflected by cell culture models. PMID: 20661576
  37. Adipophilin can be valuable in an immunohistochemical panel when evaluating cutaneous lesions with clear cell histology PMID: 20118912
  38. Adipophilin augment inflammation in macrophages, which might be one role of adipophilin in atherosclerosis. PMID: 19851831
  39. ADRP upregulation mediated by retinol and palmitate promotes downregulation of hepatic stellate cells activation and is functionally linked to the expression of fibrogenic genes. PMID: 20143336
  40. Expression patterns of perilipin and adipophilin in nonalcoholic fatty liver disease livers vary with the size of lipid droplets PMID: 20032580
  41. Findings revealed an upregulation of ADRP during macrophages differentiation into foam cells. PMID: 19351497
  42. LXR, upon stimulation with GW3965, directly regulates human ADFP transcription by binding to LXR response elements located in the 3'-untranslated and the 5'-flanking regions PMID: 19843633
  43. induction by hypoxia PMID: 12387890
  44. targeting of the lipid droplet-binding adipocyte differentiation-related protein. PMID: 12591929
  45. Expression of adipophilin is enhanced during trophoblast differentiation and is up-regulated by ligand-activated PPARgamma/retinoid X receptor. May contribute to fatty acid uptake by placenta. PMID: 14671211
  46. Stimulation of adipophilin expression in macrophages by modified LDL promotes triglyceride & cholesterol storage and reduces cholesterol efflux. Adipophilin might contribute in vivo to lipid accumulation in the intima of the arterial wall. PMID: 14707038
  47. GDP-bound ARF1 induces dissociation of ADRP from the Lipid droplet surface;. PMID: 15336557
  48. TIP47 and adipophilin are components of many, but not all, the lipid droplets in THP-1 cells PMID: 15545278
  49. ADRP and TG regulate each other, and the ubiquitin-proteasome system is involved in degradation of ADRP during regression of lipid-storing cells. PMID: 16230742
  50. Data demonstrate that adipose differentiation-related protein (ADRP) mRNA and protein are regulated by fatty acids in a human placental choriocarcinoma cell line (BeWo) and in primary human trophoblasts. PMID: 16391323
  51. We demonstrate that ADFP is transcriptionally regulated by peroxisome proliferator-activated receptor alpha (PPARalpha) in human hepatoma cells through a highly conserved direct repeat-1(DR-1) element. PMID: 16489205
  52. The results suggested that the putative hydrophobic cleft is critical for the unique characteristics of TIP47. PMID: 16808905
  53. Adipophilin expression in THP-1 macrophages altered the cellular content of different lipids and enhanced the size of lipid droplets PMID: 16884492
  54. adipose differentiation-related protein does not appear to have a role in lung surfactant production PMID: 16936283
  55. ADFP expression may have a role in clear cell renal carcinoma differentiation PMID: 17200350
  56. the metallothionein genes, adipophilin (ADFP), CD36, adipocyte fatty acid binding protein (FABP4), ATP binding cassette protein A1 (ABCA1), and liver X receptor (LXR[alpha]) all emerged as strongly positively correlated with PPAR[gamma] expression. PMID: 17322100
  57. Because adipophilin blocks cholesterol efflux from lipid-laden cells, they may die and develop a necrotic lipid core, thereby destabilizing the symptomatic carotid plaque. PMID: 17446422
  58. agLDL-LRP1 engagement induces adipocyte differentiation-related protein overexpression in both monocyte-derived macrophages and human vascular smooth muscle cells PMID: 17620659
  59. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition PMID: 17927964
  60. Mutations in known genes account for 58% of autosomal dominant retinitis pigmentosa (adRP). PMID: 18188946
  61. Adipophilin and TIP47 are expressed in lipid droplets of vitamin A-storing hepatic stellate cells and additionally in lipid droplets of steatotic hepatocytes. PMID: 18393390
  62. Increased expression by OxLDL in PMA-differentiated THP-1 macrophages and in plaque vs. nonplaque lesion areas in human carotid endarterectomy specimens (it(correlated with CD36 expression). PMID: 18827892
  63. The present results suggest that lipid droplets (LD) and LD-associated proteins have protective effects against apoptosis induced by fatty acid-bound albumin by sequestering free fatty acids. PMID: 18832575
  64. Mycobacterium leprae regulates ADRP/perilipin expression to facilitate the accumulation of lipids within infected macrophages for intracellular survival. PMID: 19054096
  65. the intramyocellular lipids (IMCL) pool is heterogeneous, as the majority but not all IMCL contain adipophilin. PMID: 19169702
  66. ADRP content was negatively associated with insulin-stimulated glucose uptake. Results indicate involvement of OXPAT and ADRP in muscular lipid accumulation and type 2 diabetes. PMID: 19602560
  67. ADFP is involved in retinoid storage and trafficking in the mouse eye. PMID: 18606814
  68. ADFP mRNA and protein are increased by long-chain polyunsaturated fatty acids in a human placental choriocarcinoma cell line (BeWo) and in primary human trophoblasts. The ADFP protein might facilitate transplacental transfer of maternal fatty acids PMID: 16391323

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Subcellular Location Membrane, Peripheral membrane protein, Lipid droplet
Protein Families Perilipin family
Tissue Specificity Milk lipid globules.
Database Links

HGNC: 248

OMIM: 103195

KEGG: hsa:123

STRING: 9606.ENSP00000276914

UniGene: Hs.3416

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