PLS1 Antibody

Datasheet
Code CSB-PA623928LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q14651
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate
    All lanes: PLS1 antibody at 5.2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 71 kDa
    Observed band size: 71 kDa

  • IHC image of CSB-PA623928LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA623928LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-PA623928LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunoprecipitating PLS1 in 293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA623928LA01HU in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA623928LA01HU (6µg) + 293 whole cell lysate (500µg)
    Lane 3: 293 whole cell lysate (20µg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Immunogen Recombinant Human Plastin-1 protein (1-143AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000
Relevance Actin-bundling protein in the absence of calcium.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Plastin-1 (Intestine-specific plastin) (I-plastin), PLS1
Immunogen Species Human
Research Area Signal Transduction
Target Names PLS1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Actin-bundling protein in the absence of calcium.
Subcellular Location Cytoplasm
Tissue Specificity In small intestine, colon, and kidney; relatively lower levels of expression are detected in the lung and stomach.
Database Links

HGNC: 9090

OMIM: 602734

KEGG: hsa:5357

STRING: 9606.ENSP00000336831

UniGene: Hs.203637

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