PMRT15 Antibody

Datasheet

Code	CSB-PA812747LA01DOA
Size	US$166
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Image	Western Blot Positive WB detected in Recombinant protein All lanes: PMRT15 antibody at 5μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana PMRT15 Polyclonal antibody

Uniprot No.

Q8GWT4

Target Names

PMRT15

Alternative Names

PMRT15 antibody; PMRT5 antibody; SKB1 antibody; At4g31120 antibody; F6E21.40Protein arginine N-methyltransferase 1.5 antibody; AtPMRT15 antibody; AtPMRT5 antibody; EC 2.1.1.319 antibody; Shk1 kinase-binding protein 1 homolog antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana Protein arginine N-methyltransferase 1.5 protein (513-637AA)

Immunogen Species

Arabidopsis thaliana

Conjugate

Non-conjugated

The PMRT15 Antibody (Product code: CSB-PA812747LA01DOA) is Non-conjugated. For PMRT15 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA812747LB01DOA	PMRT15 Antibody, HRP conjugated	ELISA
FITC	CSB-PA812747LC01DOA	PMRT15 Antibody, FITC conjugated
Biotin	CSB-PA812747LD01DOA	PMRT15 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Methylates arginine residues of myelin basic protein (MBP) in vitro. Methylates symmetrically histone H4 of the FLC chromatin to form H4R3me2s, which in turn suppresses FLC expression to induce flowering. Regulates alternative splicing by methylating spliceosomal proteins. Involved in the post-transcriptional regulation of the circadian clock.

Gene References into Functions

AtPRMT5 Regulates Shoot Regeneration through Mediating Histone H4R3 Dimethylation on KRPs and Pre-mRNA Splicing of RKP in Arabidopsis PMID: 27780782
a new mechanism of plant root stem cell maintenance under DNA damage conditions that requires SKB1 PMID: 27090604
Plants transduce stress-triggered NO signal to protein methylation machinery through S-nitrosylation of PRMT5 in response to environmental alterations. PMID: 28757206
SKB1 and SKB1-mediated H4R3sme2 are required for the maintenance of SAM in Arabidopsis seedlings. PMID: 24349476
A mutation in CAU1 significantly increased the expression level of the calcium signaling gene CAS, and functional disruption of CAS abolished the enhanced drought tolerance and stomatal [Ca(2+)]o signaling in cau1. PMID: 23943859
SKB1 methylates the U6 snRNP LSM4 in response to salt stress and ABA. Loss of SKB1 influences pre-mRNA splicing. PMID: 21258002
period lengthening associated with mutational disruption of prmt5 indicates that Rsme2 is a decoration important for the Arabidopsis clock PMID: 21097700
key role for PRMT5 in the regulation of alternative splicing PMID: 20962777
AtPRMT5 deficiency causes splicing defects in hundreds of genes involved in multiple biological processes. The splicing defects are identified in transcripts of several RNA processing factors involved in regulating flowering time. PMID: 20956294
SKB1-mediated H4R3sme2 is a novel histone mark required for repression of FLC expression and flowering time control. PMID: 17363895
involved in promotion of vegetative growth and FlOWERING LOCUS C-dependent flowering time regulation PMID: 17573539

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Subcellular Location

Cytoplasm.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family

Tissue Specificity

Highly expressed in flowers, roots and siliques, lower expression in stems and mature leaves (at protein level). Abundant in shoot apex, young leaves and leaf primordia, floral and inflorescence meristems, gynoecium, stamens, sepals and young siliques, bu

Database Links

KEGG: ath:AT4G31120

STRING: 3702.AT4G31120.1

UniGene: At.31771

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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