PNCK Antibody

Code CSB-PA754178LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue, Mouse kidney tissue
    All lanes: PNCK antibody at 3.1µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39, 41, 28, 47 kDa
    Observed band size: 39 kDa

  • IHC image of CSB-PA754178LA01HU diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PNCK Polyclonal antibody
Uniprot No.
Target Names
PNCK
Alternative Names
Calcium/calmodulin dependent protein kinase type 1B antibody; Calcium/calmodulin-dependent protein kinase type 1B antibody; CaM KI beta antibody; CaM kinase I beta antibody; CaM kinase IB antibody; CaM-KI beta antibody; CaMK1b antibody; CaMKI beta antibody; CaMKI-beta antibody; EC 2.7.11.17 antibody; KCC1B_HUMAN antibody; MGC45419 antibody; PNCK antibody; Pregnancy up regulated non ubiquitously expressed CaM kinase antibody; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant Human Calcium/calmodulin-dependent protein kinase type 1B protein (1-115AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PNCK Antibody (Product code: CSB-PA754178LA01HU) is Non-conjugated. For PNCK Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA754178LB01HU PNCK Antibody, HRP conjugated ELISA
FITC CSB-PA754178LC01HU PNCK Antibody, FITC conjugated
Biotin CSB-PA754178LD01HU PNCK Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The PNCK polyclonal antibody is a rabbit-derived antibody that has been specifically designed to target the PNCK protein. The immunogen used to generate this antibody is a recombinant human PNCK protein (1-115aa). The PNCK protein mainly acts as a serine/threonine kinase that plays a role in various cellular processes, including cell proliferation, migration, survival, and differentiation. PNCK overexpression has been detected in a variety of tumor types, including breast, ovarian, and prostate cancer, and can promote cancer cell proliferation, migration, and invasion.

This PNCK antibody has been purified using protein G affinity chromatography and has a purity of >95%. It has been validated for use in various applications, including ELISA, WB, and IHC. And it shows broad reactivity with human, mouse, and rat samples.

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Target Background

Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1.
Gene References into Functions
  1. Pnck may be a marker of Trastuzumab resistance and possibly a therapeutic target in breast cancer. PMID: 25773930
  2. the relationship between PNCK and prognosis in clear cell renal cell carcinoma PMID: 23634203
  3. Pnck induces epidermal growth factor receptor degradation, most likely through perturbation of Hsp90 chaperone activity due to Hsp90 phosphorylation. Epidermal growth factor receptor degradation is coupled to proteasomal degradation of Pnck. PMID: 21325639
  4. Pnck induces ligand-independent EGFR degradation, and it may represent an attractive therapeutic target in EGFR-regulated oncogenesis. PMID: 18562482
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
Database Links

HGNC: 13415

OMIM: 300680

KEGG: hsa:139728

STRING: 9606.ENSP00000405950

UniGene: Hs.436667

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