Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PNN Polyclonal antibody

Uniprot No.

Q9H307

Target Names

PNN

Alternative Names

140 kDa nuclear and cell adhesion-related phosphoprotein antibody; Desmosome-associated protein antibody; Domain-rich serine protein antibody; DRS antibody; DRS protein antibody; DRSP antibody; Melanoma metastasis clone A protein antibody; MEMA antibody; Nuclear protein SDK3 antibody; Pinin antibody; PININ_HUMAN antibody; PNN antibody; SR like protein antibody; SR-like protein antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthesized peptide derived from internal of Human PNN.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:3000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

Gene References into Functions

Data suggest that SNRPA1, SNRPD1, and PNN are key players in the regulation of pluripotency-specific spliceosome assembly and the acquisition and maintenance of pluripotency. PMID: 28595116
Pinin contributes to hepatocellular carcinoma progression and resistance to glucose deprivation-induced apoptosis via maintaining ERK1/2 activation PMID: 27175589
Our findings suggested that PNN, as a valuable marker of prognosis, has important influence on the progression of colorectal cancer PMID: 27107420
Pinin and CtBP are oncotargets that closely interact with each other to regulate transcription and pre-mRNA alternative splicing and promote cell adhesion and other epithelial characteristics of ovarian cancer cells. PMID: 26871283
These data suggest that lowering of PNN levels in epithelial cells results in dramatic transformation in the number and composition of splicing variants and that PNN plays a crucial role in the selection of which RNA isoforms differentiating cells produce. PMID: 26900324
PNN has an important role in alternative splicing of a specific subset of lncRNAs of the corneal epithelium. PMID: 25489234
ESRP1 and PNN modulate alternative splicing of a specific subset of target genes, but not general splicing events, in HCET cells to maintain or enhance epithelial characteristics. PMID: 23299472
Urinary desmosine level is an important biologoical marker, in patients treated with bilateral knee replacement and hydrocortison therapy. PMID: 23097096
Pinin functions as a splicing regulator which participates itself directly in splicing reaction or indirectly via other components of splicing machinery PMID: 12051732
Pnn may participate, via its interaction with RNPS1, in mRNA metabolism in the nucleus, including mRNA splicing and export PMID: 14517304
the interaction of Pnn with the corepressor CtBP1 may modulate repression of E-cadherin transcription by CtBP1 PMID: 15542832
Pnn may play a general role in the control of the cellular amount of family SR proteins through down-regulation of its own expression PMID: 16430868
Corepressor CtBP and PNN/DRS differentially modulate transcription and splicing of the E-cadherin gene. PMID: 18086895

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Subcellular Location

Nucleus speckle. Cell junction, desmosome. Note=Cell-cell contact area, predominantly desmosome of intercellular adherens junction. Not a nucleocytoplasmic shuttling protein.

Protein Families

Pinin family

Tissue Specificity

Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primar

Database Links

HGNC: 9162

OMIM: 603154

STRING: 9606.ENSP00000216832

UniGene: Hs.409965

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Code	CSB-PA255188
Size	US$297
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Image	Western blot analysis of extracts from HT-29 cells, using PNN antibody.
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PNN Antibody

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