POLE4 Antibody

Datasheet

Code	CSB-PA889087LA01HU
Size	US$166
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Image	IHC image of CSB-PA889087LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA889087LA01HU diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) POLE4 Polyclonal antibody

Uniprot No.

Q9NR33

Target Names

POLE4

Alternative Names

DNA polymerase epsilon 4 accessory subunit antibody; DNA polymerase epsilon p12 subunit antibody; DNA polymerase epsilon subunit 4 antibody; DNA polymerase epsilon subunit p12 antibody; DNA polymerase II subunit 4 antibody; DPOE4_HUMAN antibody; p12 antibody; POLE4 antibody; Polymerase (DNA directed) epsilon 4 (p12 subunit) antibody; Polymerase (DNA directed) epsilon 4 antibody; YHHQ1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DNA polymerase epsilon subunit 4 protein (8-117AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The POLE4 Antibody (Product code: CSB-PA889087LA01HU) is Non-conjugated. For POLE4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA889087LB01HU	POLE4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA889087LC01HU	POLE4 Antibody, FITC conjugated
Biotin	CSB-PA889087LD01HU	POLE4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication.

Subcellular Location

Nucleus.

Database Links

HGNC: 18755

OMIM: 607269

KEGG: hsa:56655

STRING: 9606.ENSP00000420176

UniGene: Hs.469060

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

DNA repair pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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