POLE4 Antibody

Datasheet
Code CSB-PA889087LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9NR33
Image
  • IHC image of CSB-PA889087LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA889087LA01HU diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human DNA polymerase epsilon subunit 4 protein (8-117AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC; Recommended dilution: IHC:1:200-1:500
Relevance May play a role in allowing polymerase epsilon to carry out its replication and/or repair function.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias DNA polymerase epsilon subunit 4 (EC 2.7.7.7) (DNA polymerase II subunit 4) (DNA polymerase epsilon subunit p12), POLE4
Immunogen Species Human
Research Area Others
Target Names POLE4
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function May play a role in allowing polymerase epsilon to carry out its replication and/or repair function.
Subcellular Location Nucleus
Database Links

HGNC: 18755

OMIM: 607269

KEGG: hsa:56655

STRING: 9606.ENSP00000420176

UniGene: Hs.469060

Pathway DNA repair pathway

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