Phospho-CCNC (Ser275) Antibody

Datasheet

Code	CSB-PA220960
Size	US$360
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Image	Western blot analysis of extracts from 3T3 cells (Lane 2), A549 cells (Lane 3) and Hela cells (Lane 4), using Cyclin C (Phospho-Ser275) Antibody. The lane on the left is treated with antigen-specific peptide. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin C (Phospho-Ser275) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CCNC Polyclonal antibody

Uniprot No.

P24863

Target Names

CCNC

Alternative Names

ccnc antibody; CCNC_HUMAN antibody; CycC antibody; Cyclin C antibody; Cyclin-C antibody; hSRB11 antibody; OTTHUMP00000016897 antibody; SRB11 homolog antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Peptide sequence around phosphorylation site of Serine 275(N-G-S(p)-A-N) derived from Human Cyclin C.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:1000
IHC	1:50-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.

Gene References into Functions

our results suggest that mTORC1 activation in NAFLD and insulin resistance results in down-regulation of the CDK8-CycC complex and elevation of lipogenic protein expression. PMID: 26042770
results suggest that CCNC temporarily protects SRC-2 against degradation and this event is involved in the transcriptional regulation of SRC-2 cell cycle target genes. PMID: 25986860
Cancer-mediated CDK8 point mutations (D173A and D189N) change the binding pattern of cdk8 to its partner, CycC. PMID: 24754906
cyclin-C-CDK complexes phosphorylate the Notch1 intracellular domain (ICN1) and promote ICN1 degradation PMID: 25344755
analysis of the structure-kinetic relationship of the cyclin-dependent kinase 8 (CDK8)/cyclin C (CycC) complex PMID: 23630251
2.2-A crystal structure of CDK8/CycC in complex with sorafenib; CDK8 structure reveals a unique CycC recognition helix that explains the specificity of the CDK8/CycC pair and discrimination among the highly promiscuous binding in the CDK/cyclin family PMID: 21806996
Studies establish cyclin C as a critical regulator of the G(0)/G(1) transition of human HSPCs and suggest that modulating cyclin C levels may be useful for HSC expansion and more efficient engraftment. PMID: 19967789
The present observations suggest different cellular functions of cyclin C in neurons and astrocytes in alzheimer's disease. PMID: 12600719
A cellular pool of cyclin C combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and this phosphorylation is required for cells to exit G0 efficiently. PMID: 15084261
Identification of multiple 1alpha,25(OH)2D3 response elements in the cyclin C promoter. PMID: 15863722
Physical chromosome mapping of the deleted region of chromosome 6 suggests that CCNC is a candidate tumor suppressor gene. PMID: 17089020
C2 isoform may play a presently unexplored and important role in mammalian testis and probably this isoform is the one that is mainly implicated in cell cycle regulation. PMID: 17385550
Data suggests that the primary regulation of Cyclin C by all-trans RA and Forskolin mediates some of the cell cycle control actions of these compounds. PMID: 19683536

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Subcellular Location

Nucleus.

Protein Families

Cyclin family, Cyclin C subfamily

Tissue Specificity

Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.

Database Links

HGNC: 1581

OMIM: 123838

KEGG: hsa:892

STRING: 9606.ENSP00000428982

UniGene: Hs.430646

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Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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