Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Immunofluorescence staining of Hela cells with CSB-PA010418OA28phHU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Peptide sequence around site of phospho-Ser(28) derived from human Histone H3
Raised in :
Species Reactivity :
Tested Applications :
ELISA, IF; Recommended dilution: IF:1:50-1:200
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.