Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PAK5/PAK6 Polyclonal antibody

Uniprot No.

Q9P286

Target Names

PAK5/PAK6

Alternative Names

EC 2.7.11.1 antibody; KIAA1264 antibody; MGC26232 antibody; p21 activated kinase 7 antibody; p21 protein (Cdc42/Rac)-activated kinase 7 antibody; p21(CDKN1A) activated kinase 7 antibody; p21-activated kinase 5 antibody; p21-activated kinase 7 antibody; PAK 5 antibody; PAK 7 antibody; PAK-5 antibody; PAK-7 antibody; PAK5 antibody; PAK7 antibody; PAK7_HUMAN antibody; Protein kinase PAK5 antibody; Serine/threonine protein kinase PAK 7 antibody; Serine/threonine-protein kinase PAK 7 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Peptide sequence around phosphorylation site of Serine 602/560(R-K-S(p)-L-V) derived from Human PAK5/6.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

Gene References into Functions

PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer. PMID: 30245957
Mechanistic studies demonstrated that miR-106a-5p directly bound to the 3'-UTR of the PAK5 mRNA and mediated a decrease in the protein expression of PAK5. PMID: 29072688
PAK5-mediated phosphorylation and nuclear translocation of NF-kappaB-p65 promotes breast cancer cell proliferation in vitro and in vivo PMID: 29041983
p21-activated kinase (PAK7) was identified as the putative target of miR492 in osteosarcoma (OS), and we further found a significantly inverse correlation between PAK7 and miR492 in OS specimens. PMID: 28677719
As PAK7 has been implicated in the progression of various tumor types, silencing the expression of PAK7 via pairing with miR526a may be an effective strategy in tumor suppression. PMID: 28560394
These findings indicate that aberrant PAK7 expression is associated with the occurrence of metastasis and poor clinical outcomes of human colon cancer by promoting the epithelial to mesechymal transition PMID: 27323857
Data suggest expression of ECAD (E-cadherin) in bladder cancer cell lines correlates with presence of intercellular junctions and level of PAK5 (p21-activated kinase PAK5 protein) expression; endogenous PAK5 is localized to intercellular junctions and interacts with ECAD; depletion of PAK5 expression reduces integrity of intercellular junctions; PAK5 mRNA levels are reduced in bladder cancer compared with normal controls. PMID: 28232500
model suggests PAK5 self-association interferes with AID binding to the catalytic domain, thus maintaining its high activity. PMID: 27095851
PAK7 expression is significantly downregulated in human masticatory mucosa during wound healing PMID: 28005267
PAK7 is overexpressed in human esophageal squamous cell cancer samples and correlated with lymph node metastasis. PMID: 26682509
E47 is a novel substrate of PAK5, and PAK5-mediated phosphorylation of E47 promotes epithelial-mesenchymal transition and metastasis of colon cancer. PMID: 26212009
Aurora-A induced PAK7 expression in esophageal cancer cells. PMID: 25436453
results suggest that the inhibition of PAK5 by RNA interference might efficiently suppress tumor development of glioma cells with PAK5 high expression. PMID: 25632266
miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma. PMID: 26116538
PAK7 promotes cell proliferation and tumorigenesis PMID: 25052921
It inhibits glioma cell migration and invasion potentially through the PAK5-Egr1-MMP2 signaling pathway. PMID: 24062079
PAK5 functions as an oncogenic kinase in tumor cellular regulation PMID: 24869804
Results demonstrate that PAK7 is developmentally co-expressed with another known psychosis risk gene (DISC1) suggesting a potential molecular mechanism involving aberrant synapse development and plasticity. PMID: 24474471
PAK5 is correlated to human epithelial ovarian cancer (EOC) and increased PAK5 expression promotes EOC progression. PMID: 23877225
The PAK5-Egr1-MMP2 signaling pathway is a critical regulator of cell migration and invasion in lung cancer cells. PMID: 23696025
identified three kinases with gain-of-function mutations in lung cancer, namely FGFR4, MAP3K9, and PAK5. Mutations in these kinases are activating toward the ERK pathway, and targeted depletion of the mutated kinases inhibits proliferation PMID: 23836671
PAK7 is a new hallmark of gastric cancer, PAK7 may contribute to gain of tumor growth potential, acting by affecting the expression of cell cycle regulators. PMID: 23106939
Data show that both Pak5 and constitutively active Pak4, the founding member of the Group B Paks, directly phosphorylate p120 in vitro. PMID: 20564219
PAK5 suppresses the activity of microtubule affinity-regulating kinase 2 (MARK2) toward its target, tau protein. PMID: 16014608
Nucleocytoplasmic shuttling of PAK7 regulates its antiapoptotic properties. PMID: 16581795
Study demonstrated that PAK5 expression increased significantly with malignant progression of CRC (colorectal cancer), and that PAK5 might promote CRC metastasis by regulating CRC cell adhesion and migration. PMID: 19415746

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Subcellular Location

Mitochondrion. Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily

Tissue Specificity

Predominantly expressed in brain.

Database Links

HGNC: 15916

OMIM: 608038

KEGG: hsa:57144

STRING: 9606.ENSP00000322957

UniGene: Hs.32539

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