Recombinant Human Serine/threonine-protein kinase PAK 5 (PAK5)

Code CSB-YP885798HU(A4)
MSDS
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Source Yeast
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Code CSB-EP885798HU(A4)-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP885798HU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAK5
Uniprot No.
Alternative Names
EC 2.7.11.1; KIAA1264; MGC26232 ; p21 activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A) activated kinase 7; p21-activated kinase 5; p21-activated kinase 7; PAK 5; PAK 7; PAK-5; PAK-7; PAK5; PAK7; PAK7_HUMAN; Protein kinase PAK5 ; Serine/threonine protein kinase PAK 7; Serine/threonine-protein kinase PAK 7
Species
Homo sapiens (Human)
Expression Region
1-719
Target Protein Sequence
MFGKKKKKIE ISGPSNFEHR VHTGFDPQEQ KFTGLPQQWH SLLADTANRP KPMVDPSCIT PIQLAPMKTI VRGNKPCKET SINGLLEDFD NISVTRSNSL RKESPPTPDQ GASSHGPGHA EENGFITFSQ YSSESDTTAD YTTEKYREKS LYGDDLDPYY RGSHAAKQNG HVMKMKHGEA YYSEVKPLKS DFARFSADYH SHLDSLSKPS EYSDLKWEYQ RASSSSPLDY SFQFTPSRTA GTSGCSKESL AYSESEWGPS LDDYDRRPKS SYLNQTSPQP TMRQRSRSGS GLQEPMMPFG ASAFKTHPQG HSYNSYTYPR LSEPTMCIPK VDYDRAQMVL SPPLSGSDTY PRGPAKLPQS QSKSGYSSSS HQYPSGYHKA TLYHHPSLQS SSQYISTASY LSSLSLSSST YPPPSWGSSS DQQPSRVSHE QFRAALQLVV SPGDPREYLA NFIKIGEGST GIVCIATEKH TGKQVAVKKM DLRKQQRREL LFNEVVIMRD YHHDNVVDMY SSYLVGDELW VVMEFLEGGA LTDIVTHTRM NEEQIATVCL SVLRALSYLH NQGVIHRDIK SDSILLTSDG RIKLSDFGFC AQVSKEVPKR KSLVGTPYWM APEVISRLPY GTEVDIWSLG IMVIEMIDGE PPYFNEPPLQ AMRRIRDSLP PRVKDLHKVS SVLRGFLDLM LVREPSQRAT AQELLGHPFL KLAGPPSCIV PLMRQYRHH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.
Gene References into Functions
  1. PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer. PMID: 30245957
  2. Mechanistic studies demonstrated that miR-106a-5p directly bound to the 3'-UTR of the PAK5 mRNA and mediated a decrease in the protein expression of PAK5. PMID: 29072688
  3. PAK5-mediated phosphorylation and nuclear translocation of NF-kappaB-p65 promotes breast cancer cell proliferation in vitro and in vivo PMID: 29041983
  4. p21-activated kinase (PAK7) was identified as the putative target of miR492 in osteosarcoma (OS), and we further found a significantly inverse correlation between PAK7 and miR492 in OS specimens. PMID: 28677719
  5. As PAK7 has been implicated in the progression of various tumor types, silencing the expression of PAK7 via pairing with miR526a may be an effective strategy in tumor suppression. PMID: 28560394
  6. These findings indicate that aberrant PAK7 expression is associated with the occurrence of metastasis and poor clinical outcomes of human colon cancer by promoting the epithelial to mesechymal transition PMID: 27323857
  7. Data suggest expression of ECAD (E-cadherin) in bladder cancer cell lines correlates with presence of intercellular junctions and level of PAK5 (p21-activated kinase PAK5 protein) expression; endogenous PAK5 is localized to intercellular junctions and interacts with ECAD; depletion of PAK5 expression reduces integrity of intercellular junctions; PAK5 mRNA levels are reduced in bladder cancer compared with normal controls. PMID: 28232500
  8. model suggests PAK5 self-association interferes with AID binding to the catalytic domain, thus maintaining its high activity. PMID: 27095851
  9. PAK7 expression is significantly downregulated in human masticatory mucosa during wound healing PMID: 28005267
  10. PAK7 is overexpressed in human esophageal squamous cell cancer samples and correlated with lymph node metastasis. PMID: 26682509
  11. E47 is a novel substrate of PAK5, and PAK5-mediated phosphorylation of E47 promotes epithelial-mesenchymal transition and metastasis of colon cancer. PMID: 26212009
  12. Aurora-A induced PAK7 expression in esophageal cancer cells. PMID: 25436453
  13. results suggest that the inhibition of PAK5 by RNA interference might efficiently suppress tumor development of glioma cells with PAK5 high expression. PMID: 25632266
  14. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma. PMID: 26116538
  15. PAK7 promotes cell proliferation and tumorigenesis PMID: 25052921
  16. It inhibits glioma cell migration and invasion potentially through the PAK5-Egr1-MMP2 signaling pathway. PMID: 24062079
  17. PAK5 functions as an oncogenic kinase in tumor cellular regulation PMID: 24869804
  18. Results demonstrate that PAK7 is developmentally co-expressed with another known psychosis risk gene (DISC1) suggesting a potential molecular mechanism involving aberrant synapse development and plasticity. PMID: 24474471
  19. PAK5 is correlated to human epithelial ovarian cancer (EOC) and increased PAK5 expression promotes EOC progression. PMID: 23877225
  20. The PAK5-Egr1-MMP2 signaling pathway is a critical regulator of cell migration and invasion in lung cancer cells. PMID: 23696025
  21. identified three kinases with gain-of-function mutations in lung cancer, namely FGFR4, MAP3K9, and PAK5. Mutations in these kinases are activating toward the ERK pathway, and targeted depletion of the mutated kinases inhibits proliferation PMID: 23836671
  22. PAK7 is a new hallmark of gastric cancer, PAK7 may contribute to gain of tumor growth potential, acting by affecting the expression of cell cycle regulators. PMID: 23106939
  23. Data show that both Pak5 and constitutively active Pak4, the founding member of the Group B Paks, directly phosphorylate p120 in vitro. PMID: 20564219
  24. PAK5 suppresses the activity of microtubule affinity-regulating kinase 2 (MARK2) toward its target, tau protein. PMID: 16014608
  25. Nucleocytoplasmic shuttling of PAK7 regulates its antiapoptotic properties. PMID: 16581795
  26. Study demonstrated that PAK5 expression increased significantly with malignant progression of CRC (colorectal cancer), and that PAK5 might promote CRC metastasis by regulating CRC cell adhesion and migration. PMID: 19415746

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Subcellular Location
Mitochondrion. Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
Tissue Specificity
Predominantly expressed in brain.
Database Links

HGNC: 15916

OMIM: 608038

KEGG: hsa:57144

STRING: 9606.ENSP00000322957

UniGene: Hs.32539

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