Phospho-RPS6KA4 (Thr568) Antibody

Code CSB-PA699329
Size US$360
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Image
  • Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using MSK2 (Phospho-Thr568) antibody. The lane on the right is treated with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSK2 (Phospho-Thr568) antibody. The picture on the right is treated with the synthesized peptide.
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RPS6KA4 Polyclonal antibody
Uniprot No.
Target Names
RPS6KA4
Alternative Names
90 kDa ribosomal protein S6 kinase 4 antibody; EC 2.7.11.1 antibody; KS6A4_HUMAN antibody; Mitogen and stress activated protein kinase 2 antibody; Nuclear mitogen and stress activated protein kinase 2 antibody; Nuclear mitogen- and stress-activated protein kinase 2 antibody; Ribosomal protein kinase B antibody; Ribosomal protein S6 kinase 90kD polypeptide 4 antibody; Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody; Ribosomal protein S6 kinase alpha 4 antibody; Ribosomal protein S6 kinase alpha-4 antibody; RPS6KA4 antibody; RSKB antibody; S6K-alpha-4 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 568 (M-Q-T(p)-P-C) derived from Human MSK2.
Immunogen Species
Homo sapiens (Human)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:3000
IHC 1:50-1:100
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
Gene References into Functions
  1. Two novel DMRs, located in RPS6KA4/MIR1237 and the AURKC promoter, were found to be hypermethylated in WT PMID: 28930610
  2. KSHV activates the MSK1/2-CREB1 pathway during primary infection and that it depends on this pathway for viral lytic replication. I PMID: 26109721
  3. Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
  4. MSK1 and MSK2 are required for maximal TFF 1 induction. PMID: 23675462
  5. MSK2 functions as an adaptor in mediating PKR activation, apparently independent of its catalytic activity. PMID: 21385567
  6. MSK1 and MSK2 are differentially regulated by CK2 during the UV response and that MSK2 is the major protein kinase responsible for the UV-induced phosphorylation of p65 at Ser(276) that positively regulates NF-kappaB activity in MDA-MB-231 cells PMID: 19933278
  7. Dimethylfumarate specifically inhibits MSK1 and 2 activations and subsequently inhibits NF-kappaB-induced gene-transcriptions, which are believed to be important in the pathogenesis of psoriasis PMID: 17495961

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Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily
Database Links

HGNC: 10433

OMIM: 603606

KEGG: hsa:8986

STRING: 9606.ENSP00000333896

UniGene: Hs.105584

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