RPS6KA4 (Ab-568) Antibody

Datasheet

Code	CSB-PA903004
Size	US$297
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Image	Western blot analysis of extracts from RAW264.7 cells, treated with UV (5mins), using MSK2 (Ab-568) antibody.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RPS6KA4 Polyclonal antibody

Uniprot No.

O75676

Target Names

RPS6KA4

Alternative Names

90 kDa ribosomal protein S6 kinase 4 antibody; EC 2.7.11.1 antibody; KS6A4_HUMAN antibody; Mitogen and stress activated protein kinase 2 antibody; Nuclear mitogen and stress activated protein kinase 2 antibody; Nuclear mitogen- and stress-activated protein kinase 2 antibody; Ribosomal protein kinase B antibody; Ribosomal protein S6 kinase 90kD polypeptide 4 antibody; Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody; Ribosomal protein S6 kinase alpha 4 antibody; Ribosomal protein S6 kinase alpha-4 antibody; RPS6KA4 antibody; RSKB antibody; S6K-alpha-4 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthesized non-phosphopeptide derived from Human MSK2 around the phosphorylation site of threonine 568 (M-Q-T(p)-P-C).

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:3000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

Gene References into Functions

Two novel DMRs, located in RPS6KA4/MIR1237 and the AURKC promoter, were found to be hypermethylated in WT PMID: 28930610
KSHV activates the MSK1/2-CREB1 pathway during primary infection and that it depends on this pathway for viral lytic replication. I PMID: 26109721
Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
MSK1 and MSK2 are required for maximal TFF 1 induction. PMID: 23675462
MSK2 functions as an adaptor in mediating PKR activation, apparently independent of its catalytic activity. PMID: 21385567
MSK1 and MSK2 are differentially regulated by CK2 during the UV response and that MSK2 is the major protein kinase responsible for the UV-induced phosphorylation of p65 at Ser(276) that positively regulates NF-kappaB activity in MDA-MB-231 cells PMID: 19933278
Dimethylfumarate specifically inhibits MSK1 and 2 activations and subsequently inhibits NF-kappaB-induced gene-transcriptions, which are believed to be important in the pathogenesis of psoriasis PMID: 17495961

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Subcellular Location

Nucleus.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily

Database Links

HGNC: 10433

OMIM: 603606

KEGG: hsa:8986

STRING: 9606.ENSP00000333896

UniGene: Hs.105584

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Protein Phosphorylation: The Dynamic Language of Cellular Regulation Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

MAPK signaling pathway TNF signaling pathway MAPK Erk pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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