Phospho-SREBF1 (S439) Antibody

Code CSB-PA050140
Size US$167
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Product Details

Uniprot No. P36956
Target Names SREBF1
Alternative Names ADD 1 antibody; bHLHd1 antibody; Class D basic helix-loop-helix protein 1 antibody; D630008H06 antibody; Processed sterol regulatory element-binding protein 1 antibody; SRBP1_HUMAN antibody; SREBF 1 antibody; SREBF1 antibody; SREBP 1 antibody; SREBP 1c antibody; SREBP-1 antibody; SREBP1 antibody; Sterol regulatory element binding protein 1 antibody; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody; Sterol regulatory element binding transcription factor 1 antibody; Sterol regulatory element-binding transcription factor 1 antibody
Raised in Rabbit
Species Reactivity Human,Rat
Immunogen Synthesized peptide derived from Human SREBP-1 around the phosphorylation site of S439.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
Gene References into Functions
  1. results reveal that nBP1a/PKM2 interaction activates lipid metabolism genes in cancer cells and that Thr-59 phosphorylation of SREBP-1a plays an important role in cancer cell proliferation. PMID: 29514980
  2. GTEE also downregulated the expression of AR and prostate-specific antigen (PSA) in both androgen-responsive and castration-resistant PCa cells. By blocking the SREBP-1/AR axis, GTEE suppressed cell growth and progressive behaviors, as well as activating the caspase-dependent apoptotic pathway in PCa cells PMID: 30301150
  3. SREBP1 trans-activates CYP24A1 expression through SREBP binding elements present in the promoter. PMID: 29653103
  4. Berberine (BBR), an effective suppressor of SREBP1 and lipogenesis regulated through reactive oxygen species (ROS)/AMPK pathway, selectively inhibited the growth of G-R nonsmall cell lung cancer cells and rheumatoid arthritis patiens but not that of normal cells PMID: 28665143
  5. These findings suggest that SREBP-1c serves as a molecular bridge between lipid metabolism and cell cycle control inclear cell renal cell carcinoma tumorigenesis. PMID: 29138263
  6. findings showed that PTEN inhibits HBV replication as well as HBV HCV co-replication. SREBP-1 is involved in HBV HCV replication inhibition by PTEN PMID: 29803738
  7. FTO increased the lipid accumulation in hepatocytes by increasing nuclear translocation of SREBP1c and SREBP1c maturation, thus improving the transcriptional activity of lipid droplet-associated protein CIDEC. PMID: 29486327
  8. common SNPs (rs62064119, rs2297508, rs11868035 and rs13306741) in the SREBP-1c gene were selected and genotyped in 593 Han patients with NAFLD and 593 healthy controls. No significant differences in genotype and allele frequencies of these four SNPs were found between cases and controls, suggesting that the SNPs are not associated with risk of NAFLD in the Chinese Han population. PMID: 27572914
  9. Data suggests that expression of CYP4F2 is down-regulated in liver of mice with non-alcoholic fatty liver disease after high-fat/Western diet and in human hepatocyte cell line exposed to excess palmitic acid, oleic acid, or fructose. Two other genes are down-regulated, PPAR gamma and SREBP-1. (CYP4F2 = cytochrome P450 family 4 subfamily F member 2; PPAR = peroxisome proliferator-activated receptor ) PMID: 28628909
  10. LncARSR promotes hepatic lipogenesis via Akt/SREBP-1c pathway and contributes to the pathogenesis of nonalcoholic steatohepatitis. PMID: 29555473
  11. CpG sites located in SREBF2 gene showed differential methylation in association with lipid traits. The expression of SREBF1 gene was inversely associated with methylation of its corresponding CpGs. Genetic variants in SREBF1 were also associated with lipid profile. SREBF1 expression was directly associated with HDL cholesterol. PMID: 28173150
  12. Epidermal growth factor receptor (EGFR) signaling enhances miR-29 expression in glioblastoma cells via upregulation of Sterol regulatory element binding protein PMID: 27477273
  13. Our finding reveals a crucial roles for SREBP1 in lipid desaturation of ccRCC through regulation of NF-kappaB signaling, which provides not only new insights in regulatory mode of NF-kappaB signaling but also a novel target for potential metabolic therapies. PMID: 29183723
  14. Our results suggest that relatively common genetic variants in stearoyl CoA desaturase and SREBF1 attenuated the positive associations between intake of a traditional diet rich in n-3 polyunsaturated fatty acids and increases in fasting cholesterol and HbA1c levels, as well as the waist-to-hip ratio among Yup'ik participants. PMID: 27467133
  15. changes in distinct lipid ratios may converge on ARF1 to increase SBP-1/SREBP-1 activity. PMID: 27320911
  16. Variants in the TOM1L2/SREBF1 locus exert opposing effects of total-body lean mass (TB-LM) and total-body less head bone mineral density (TBLH-BMD) . PMID: 28743860
  17. Date indicate that sterol regulatory element-binding proteins Srebp1 and Srebp2 are essential for the metabolic reprogramming of NK cells and for the attainment of elevated glycolysis and oxidative phosphorylation. PMID: 28920951
  18. Study identified a novel human specific lncRNA, lncHR1, as a negative regulator of SREBP-1c expression. Overexpression of lncHR1 inhibited expression of SREBP-1c and fatty acid synthase (FAS) and then repressed oleic acid-induced hepatic cell triglyceride (TG) and lipid droplet (LD) accumulation. PMID: 28367099
  19. Glucose adsorption to chitosan membranes increases proliferation of human chondrocytes via mammalian target of rapamycin complex 1 and sterol regulatory element-binding protein-1 signaling. PMID: 28218386
  20. miR-185 negatively regulates the differentiation of 3T3-L1 cells by targeting SREBP-1 PMID: 28701079
  21. The authors further demonstrated that the upregulation of sterol regulatory element-binding protein (SREBP)-1c by activation of the Akt and p70S6K pathways is critical for high-glucose-treated Porphyromonas gingivalis-induced NLRP3 expression. PMID: 28083517
  22. Results show that PPARalpha is downregulated and SREBP-1c is upregulated in steatosis L-02 cells. These changes increase lipid synthesis and reduce lipid disposal, which ultimately lead to hepatic steatosis. PMID: 27270405
  23. SREBP-1 and SREBP-2 mRNA expression levels were measured in EAT from 49 patients with CAD (26 with diabetes) and 23 controls without CAD or diabetes.SREBP expression was associated as cardiovascular risk factor for the severity of CAD and the poor lipid control. PMID: 28367087
  24. The involvement of SREBP-1c in FASN promoter histone modification. PMID: 28027934
  25. the mitotic phosphorylation and stabilization of nuclear SREBP1 during cell division provides a link between lipid metabolism and cell proliferation. PMID: 27579997
  26. B7-H3 hijacks SREBP-1/FASN signaling mediating abnormal lipid metabolism in lung cancer PMID: 27939887
  27. The genetic polymorphisms of SREBF1 could play a role in the mechanism for interindividual variation of atypical antipsychotics-induced metabolic syndrome (MetS). SCAP polymorphisms with drug-induced MetS were negative in this study. PMID: 26982812
  28. NS5ATP6 regulates the intracellular triglyceride level via FGF21, and independently of SIRT1 and SREBP1. PMID: 27179781
  29. observations suggest that MALAT1 promotes hepatic steatosis and insulin resistance by increasing nuclear SREBP-1c protein stability. PMID: 26935028
  30. MiR-132 inhibited SIRT1 and SREBP-1c expression and downregulated their targeted genes, including HMGCR and FASN. PMID: 26898440
  31. Report demonstrated that overexpression of SULT2B1b-mediated angiogenic signaling was associated with tumor angiogenesis and poor clinical features of human gastric cancer. PMID: 26937945
  32. Data show that mutant p53 protein activates the sterol regulatory element-binding proteins SREBP-1 and SREBP-2-mediated signaling pathways in prostate cancer (PCa) cells. PMID: 26512780
  33. miR-33b is highly induced upon differentiation of human preadipocytes, along with SREBP-1 and miR-33b is an important regulator of adipogenesis PMID: 26830228
  34. Akt1 and Akt2 activated both SREBP-1 and SREBP-2, whereas Akt3 upregulated SREBP-1 to enhance hepatitis C virus translation. PMID: 26855332
  35. PRMT5-induced methylation prevented phosphorylation of SREBP1a on S430 by GSK3beta PMID: 26759235
  36. mTORC2 positively regulates mSREBP1 stability and lipogenesis. Findings reveal a novel biological function of mTORC2 in the regulation of lipogenesis PMID: 25893295
  37. mTORC1/SREBP pathway is a major mechanism through which common oncogenic signaling events induce de novo lipid synthesis to promote aberrant growth and proliferation of cancer cells PMID: 26028026
  38. hnRNP A1 is implicated in the free fatty acid-induced expression of SREBP-1a and of its target genes as well as in the lipid accumulation in hepatocytes. PMID: 26869449
  39. TG levels are regulated by HCBP6-sterol regulatory element binding protein 1c (SREBP1c)-mediated fatty acid synthase (FASN) expression. PMID: 25855506
  40. Aberrant activation of SREBP1c suppresses primary ciliogenesis by PLA2G3-mediated distortion of vesicular trafficking and suggest that PLA2G3 is a novel potential target to normalize ciliogenesis in SREBP1c-overexpressing cells, including cancer cells. PMID: 25904332
  41. PD-L1 induces epithelial-to-mesenchymal transition via activating SREBP-1c in renal cell carcinoma. PMID: 26141060
  42. performed a detailed promoter/enhancer analysis of ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1 PMID: 26321664
  43. Data indicate that glucose-mediated glycosylation promotes SREBP cleavage-activating protein (SCAP) trafficking to the Golgi Leading to sterol regulatory element binding protein 1 (SREBP-1) activation. PMID: 26555173
  44. Single nucleotide polymorphism (rs2297508) of SREBF-1 may serve as a genetic predisposition factor for the development of endometrial cancer. PMID: 24614076
  45. Report PCR techniques for genotyping SREBF1 rs8066560 variant in Iranian children/adolescents with metabolic syndrome. PMID: 26771965
  46. Data show that the cleavage site of the lipid-signaling protein sterol regulatory element binding transcription factor 1 (SREBP-1) intermediate bears rigid alpha-helical topology. PMID: 26392539
  47. We concluded that 54(G/C) polymorphism of SREBF-1 gene is associated with polycystic ovary syndrome (PCOS) and suggest that SREBF-1 gene may play a role in genetic predisposition to PCOS. PMID: 25801724
  48. The results from this study demonstrate that metformin ameliorates PA-induced insulin resistance through the activation of AMPK and the suppression of SREBP-1c in skeletal muscle cells. PMID: 25891779
  49. Associations between triglyceride levels and SREBF1 and ABCG1 were also found in adipose tissue of the Multiple Tissue Human Expression Resource cohort PMID: 25583993
  50. gene expression analysis revealed that SREBP defines a gene signature that is associated with poor survival in glioblastoma PMID: 25619842
  51. Mutated A-type lamins deregulates SREBP1 activity in Dunnigan-type familial partial lipodystrophy. PMID: 25524705
  52. Methyl protodioscin increases ABCA1 expression and cholesterol efflux while inhibiting gene expressions for synthesis of cholesterol and triglycerides by suppressing SREBP transcription and microRNA 33a/b levels. PMID: 25733328
  53. Data show that microRNA-33b (miR-33b) is co-expressed with sterol regulatory element-binding protein 1 (SREBP1) in HepG2 cells. PMID: 24931346
  54. Our results indicate that SREBP1 had important role in tumor progression and appears to be a novel prognostic marker for pancreatic cancer. PMID: 25589463
  55. Results showed that RNA isoform SREBP1a is significantly contributing to the human hepatic SREBP1 pool and has a share in human hepatic lipogenic gene expression. PMID: 25634759
  56. The data indicate a direct inhibitory interaction of polyunsaturated fatty acids with LXRalpha, a consequent reduction of SREBP-1 and of its binding to SCD1 promoter. PMID: 25264165
  57. nuclear HBx had stronger effects on SREBP-1a and fatty acid synthase transcription activation, intracellular lipid accumulation and cell proliferation PMID: 25800871
  58. It is inferred that treatment of human neutrophils with oleic acid and an liver X receptor agonist results in a differential effect on the promoter activities of ABCA1 and SREBP1c. PMID: 24722912
  59. These results demonstrate that SREBP-1c is a direct activator of the human PNPLA3 gene and insulin transactivates the PNPLA3 gene via the PI3K-SREBP-1c/NFY pathway in HepG2 cells PMID: 25655569
  60. Knockdown of SIRT1 could downregulate expression of SREBP1 and suppress cell proliferation. These results demonstrated that SIRT1 may play a role as a tumor promoter in EC and can promote endometrial tumor growth by promoting lipogenesis PMID: 25270091
  61. phosphorylation of sterol regulatory element-binding protein (SREBP)-1c by p38 kinases, ERK and JNK PMID: 25353341
  62. SREBP-1c cleavage is subjected to a novel PUFA-regulated cleavage system in addition to the sterol-regulatory SCAP/Insig system. PMID: 24729033
  63. MiR132 inhibits expression of SIRT1 and induces pro-inflammatory processes of vascular endothelial inflammation through blockade of the SREBP-1c metabolic pathway PMID: 24924687
  64. Genetic variability within SREBF1 gene has an impact on the insulin sensitivity in response to a fish oil supplementation. PMID: 25270430
  65. identified interaction of three genes in INSIG-SCAP-SREBP pathway on risk of obesity, revealing that these genes affect obesity more likely through a complex interaction pattern than single gene effect. PMID: 25028659
  66. We found that rs8066560 variant on SREBP-1 and miR-33b genes is associated with LDL-C levels; however, it may not be an important determinant for the MetS, hyperglycemia, and insulin resistance in children and adolescents. PMID: 24825092
  67. Elaidic acid increases hepatic lipogenesis by mediating SREBP-1c activity in HuH-7 cells, demonstrating a unique role of elaidic acid, the most abundant trans fatty acid, in modulating hepatic lipogenesis PMID: 24481861
  68. Docosahexaenoic acid may protect against obesity-associated inflammation and colon carcinogenesis by suppressing insulin-induced activation of SREBP-1 and expression of COX-2 through up-regulation of SIRT1. PMID: 25199460
  69. In vitro studies revealed that downregulation of SREBP-1 inhibited cell proliferation and induced apoptosis in both HepG2 and MHCC97L cells (p < 0.05). PMID: 24776759
  70. Uric acid induces triglyceride accumulation by SREBP-1c activation via induction of endoplasmic reticulum stress in hepatocytes. PMID: 25111690
  71. Data suggest that sterol regulatory element binding transcription factor 1 (SREBF1), may contribute to sporadic Parkinson disease (PD) and further underscore the impact of dysfunctional mitophagy on pathogenesis. PMID: 24912190
  72. Rating SREBF1 polymorphism did not reveal a relationship to the occurrence of ischemic heart disease in patients with obstructive sleep apnea, both in the whole group as well as separate subgroups. PMID: 24868893
  73. Sterol regulatory element-binding protein-1/FASN is involved in the proliferation suppression and apoptosis promotion brought about by megestrol acetate in Ishikawa cells. PMID: 24105476
  74. significant association between SREBP-1a and cell proliferation in human preadipocytes. SREBP-1a could regulate cell cycle progression by modulating expression of potential new SREBP-1a cell cycle-related gene targets PMID: 24704425
  75. Increased SREBP1 expression is associated with ovarian cancer. PMID: 23818099
  76. Our results indicated that the dysregulation of INSIG1 and SREBF1 caused by ART were observed not only in the fetus but also in the placenta, primarily in the ICSI group PMID: 24484994
  77. Inhibition of PP2A by okadaic acid curtailed the free fatty acids induced upregulation of SREBP1 expression, fatty acid synthase promoter transcriptional activity and lipid accumulation in HepG2 cells. PMID: 23184344
  78. Carriers of both PNPLA3 and SREBP1c risk genotypes displayed significantly (p = 0.005) higher median liver stiffness. PMID: 24152445
  79. Both FOXO1 and SREBP1c overexpression converged on upregulation of glucokinase, providing substrates for DNL, in NASH patients. PMID: 23147115
  80. The SF1 and SREBP1 activate DGKtheta; transcription in a cAMP-dependent manner in human adrenocortical cells. PMID: 23610160
  81. Raising plasma insulin to postprandial levels, increased SREBP-1 mRNA comparably in livers and epidydimal adipose tissue of alert, free moving rats and in adipose tissue of human subjects. PMID: 23913732
  82. SREBP-1c silencing reduced triglycerides and downregulated fatty acid synthase expression. PMID: 23703028
  83. dysregulation of SIRT1-AMPK-SREBP and stimulation of NLRP3 inflammasome may contribute to vascular lipid deposition and inflammation in atherosclerosis PMID: 23825667
  84. S-allyl cysteine decreased protein expression levels of SREBP-1c and fatty acid synthase in free fatty acid-induced HepG2 cells. PMID: 23465592
  85. PGC-1b, a coactivator of both LXR-alpha and SREBP-1, was markedly down-regulated by OEPAs compared with EPA. PMID: 23680128
  86. Lipogenic genes are regulated by SREBP1 in ductal carcinoma in situ of breast cancer. PMID: 23208501
  87. sterol regulatory element binding transcription factor 1 (SREBF-1c) polymorphism is associated with increased risk of developing nonalcoholic fatty liver disease with more severe liver histology and derangement in glucose and lipoprotein metabolism PMID: 23985808
  88. SREBP-1c is a novel insulin/JNK2-regulated gene and that the JNK2/SREBP-1c pathway mediates insulin-induced fatty acid synthesis, which may lead to enlargement of LDs in human adipocytes. PMID: 23515281
  89. These results demonstrated that HBx activates SREBP-1a activity at the transcription level through a complex mechanism involving two bZIP transcription factors C/EBP and E4BP4 with C/EBP being the dominant positive factor. PMID: 23422505
  90. SREBP-1 regulate muscle protein synthesis through the downregulation of the expression of MYOD1, MYOG and MEF2C factors. PMID: 23226416
  91. increased postprandial stearic acid is caused by the insulin-stimulated SREBP-1c pathway and elongation of palmitic acid in hyperlipidemia. PMID: 22966071
  92. Data indicate that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) participates in the IRES (internal ribosome entry site) -dependent translation of sterol-regulatory-element-binding protein 1 (SREBP-1a) mRNA through RNA-protein interaction. PMID: 23106379
  93. The regulation of SREBP-1c gene expression by SIRT1 may require the deacetylation of LXR transcription factors. PMID: 22984430
  94. Suppression of SREBP-1 leads to FASN reduction and hypoxic cell death. PMID: 22786746
  95. ERLIN2 regulates activation of SREBP (sterol regulatory element-binding protein) 1c, the key regulator of de novo lipogenesis, in cancer cells PMID: 22690709
  96. EGFRvIII can promote tumor survival through PI3K/SREBP-1-dependent upregulation of LDLR. PMID: 22059152
  97. SREBP1 is essential for EC cell growth both in vitro and in vivo, suggesting that SREBP1 activity may be a novel therapeutic target for endometrial cancers. PMID: 22672904
  98. The mRNA levels of LXRalpha and SREBP-1c, transcription factors that regulate SCD-1, were decreased by 10,12 conjugated linoleic acid in human adipocytes. PMID: 21775116
  99. The genotypic and allelic frequencies of sterol regulatory element binding protein-1c rs2297508 and rs11868035 polymorphisms did not differ significantly in the control groups of Han and Dongxiang ethnics (P> 0.05). PMID: 22678801
  100. Data suggest that bile acids down-regulate Cav1 expression by inhibition of proteolytic cleavage of 125-kDa pre-SREBP1 in endoplasmic reticulum/Golgi apparatus and by inhibition of nuclear translocation of active 68-kDa SREBP1. PMID: 22474125

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Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein, Golgi apparatus membrane, Multi-pass membrane protein, Cytoplasmic vesicle, COPII-coated vesicle membrane, Multi-pass membrane protein
Protein Families SREBP family
Tissue Specificity Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma
Database Links

HGNC: 11289

OMIM: 184756

KEGG: hsa:6720

STRING: 9606.ENSP00000348069

UniGene: Hs.592123

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