RABEP1 Antibody

Datasheet

Code	CSB-PA614886LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: RABEP1 antibody at 1:2500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 100, 96 kDa Observed band size: 100 kDa IHC image of CSB-PA614886LA01HU diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RABEP1 Polyclonal antibody

Uniprot No.

Q15276

Target Names

RABEP1

Alternative Names

RABEP1 antibody; RAB5EP antibody; RABPT5 antibody; RABPT5A antibody; Rab GTPase-binding effector protein 1 antibody; Rabaptin-4 antibody; Rabaptin-5 antibody; Rabaptin-5alpha antibody; Renal carcinoma antigen NY-REN-17 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Rab GTPase-binding effector protein 1 protein (1-255AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RABEP1 Antibody (Product code: CSB-PA614886LA01HU) is Non-conjugated. For RABEP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA614886LB01HU	RABEP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA614886LC01HU	RABEP1 Antibody, FITC conjugated
Biotin	CSB-PA614886LD01HU	RABEP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium.

Gene References into Functions

SH2B1 and RABEP1 genetic variants are associated with worsening of LDL and glucose parameters in patients treated with psychotropic drugs PMID: 28694205
The results contradict the model of feedback activation of Rab5 and instead indicate that Rbpt5 is recruited by both Rabex5 recognizing ubiquitylated cargo and by Rab4 to activate Rab5 in a feed-forward manner. PMID: 26430212
ITSN2L interacts with RABEP1 and stimulates its degradation in regulation of endocytosis. PMID: 26633357
The pleckstrin homology domain (PH) of PLD1 itself promotes degradation of HIF-1alpha, then accelerates EGFR endocytosis via upregulation of rabaptin-5 and suppresses tumor progression. PMID: 26680696
analysis of Rabex-5 GEF activation by Rabaptin-5 PMID: 24957337
The disruption of Rabaptin-5 Ser407 phosphorylation reduces persistent cell migration in 2D and alphavbeta3-dependent invasion. PMID: 22975325
Silencing of Rabaptin-5 induces down-regulation of recycling of K(V)10.1 channel in transfected cells and reduction of K(V)10.1 current density in cells natively expressing K(V)10.1, indicating a role of Rabaptin-5 in channel trafficking. PMID: 22841712
Breast cancer cell line studies showed that microRNA, miR-373, was capable of promoting breast cancer invasion and metastasis via translational inhibition of TXNIP and RABEP1 that were the direct target genes of miR-373. PMID: 21271679
GGAs, a family of Arf-dependent clathrin adaptors involved in selection of TGN cargo, interact with the Rabaptin-5-Rabex-5 complex, a Rab4/Rab5 effector regulating endosome fusion PMID: 12505986
The authors determined that increased listeriolysin O-independent dissolution of vacuoles during RABEP1 knockdown required the Listeria monocytogenes broad-range phospholipase C (PC-PLC). PMID: 19500109

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Subcellular Location

Cytoplasm. Early endosome. Recycling endosome. Cytoplasmic vesicle.

Protein Families

Rabaptin family

Database Links

HGNC: 17677

OMIM: 603616

KEGG: hsa:9135

STRING: 9606.ENSP00000262477

UniGene: Hs.584784

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis

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