RAMP2 Antibody

Datasheet

Code	CSB-PA019305LA01HU
Size	US$166
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Image	IHC image of CSB-PA019305LA01HU diluted at 1:500 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA019305LA01HU at 1:230, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RAMP2 Polyclonal antibody

Uniprot No.

O60895

Target Names

RAMP2

Alternative Names

RAMP2; Receptor activity-modifying protein 2; Calcitonin-receptor-like receptor activity-modifying protein 2; CRLR activity-modifying protein 2

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Receptor activity-modifying protein 2 protein (43-145AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RAMP2 Antibody (Product code: CSB-PA019305LA01HU) is Non-conjugated. For RAMP2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA019305LB01HU	RAMP2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019305LC01HU	RAMP2 Antibody, FITC conjugated
Biotin	CSB-PA019305LD01HU	RAMP2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL.

Gene References into Functions

Single nucleotide polymorphism of RAMP2 is associated with Stroke. PMID: 28904253
This work suggests that RAMP2 may modify the agonist activity and trafficking of the GCGR, with potential relevance to production of new peptide analogs with selective agonist activities. PMID: 28586439
Data suggest that a single GlcNAc residue at CTR N130 (asparagine 130) is responsible for enhanced affinity of calcitonin for CTR ECD; the same appears to apply for enhanced affinity of amylin for RAMP2-CTR ECD. [GlcNAc = N-acetylglucosamine; CTR = calcitonin receptor; ECD = extracellular domain; RAMP2 = receptor (calcitonin) activity modifying protein 2]. PMID: 28614667
This study reveals the glucagon receptor as a previously unidentified target for GLP-1 receptor agonists and highlights a role for RAMP2 in regulating its pharmacology. PMID: 26198634
Adrenomedullin-RAMP2 system suppresses ER stress-induced tubule cell death and is involved in kidney protection. PMID: 24505304
Data suggest isoforms of RAMP modulate accessibility of peptides to residues situated on CALCRL (calcitonin receptor-like receptor) N-terminal domain; RAMP3/RAMP2/RAMP1 appear to alter accessibility of specific residues at CALCRL-RAMP interface. PMID: 24199627
RAMP2 gene expression increases with gestational age development in the fetal lung. PMID: 24169318
Co-expression of calcitonin receptors (CT) lacking a portion of domain 1 with receptor activity-modifying protein (RAMP) 1, 2, or 3 appears to produce functional CT-(8-32)-sensitive adrenomedullin receptors. PMID: 12565884
TNF-alpha induced time- and dose-dependent decreases in the expression of RAMP2 mRNA in cultured human coronary artery smooth muscle cells , thereby diminishing AM-evoked cAMP production PMID: 15245870
Data found that expressions of RAMP1, RAMP2 and RAMP3 mRNAs increased with the worsening of heart function, but the expressions of RAMP1 and RAMP2 mRNA decreased at level IV of heart failure. PMID: 15300632
adrenomedullin receptors are comprised of RAMP2 and calcitonin receptor-like receptor. PMID: 15613468
This study reveals important functionality of the RAMP C-terminal domain and identifies key differences in the role of the RAMP C terminus for calcitonin receptor versus calcitonin receptor-like receptor-based receptors. PMID: 16912219
RAMP2 is silenced by promoter hypermethylation in lung cancer PMID: 17671114
Identification of RAMP2 residues for adrenomedullin receptors are reported. PMID: 18593822
The His residues of hRAMP2 and -3 differentially govern adrenomedullin receptor function. PMID: 18835256

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

RAMP family

Tissue Specificity

Strongly expressed in lung, breast, immune system and fetal tissues.

Database Links

HGNC: 9844

OMIM: 605154

KEGG: hsa:10266

STRING: 9606.ENSP00000253796

UniGene: Hs.514193

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Vascular smooth muscle contraction

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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