RFNG Antibody

Code CSB-PA896922LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissue
    All lanes: RFNG antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • IHC image of CSB-PA896922LA01HU diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RFNG Polyclonal antibody
Uniprot No.
Target Names
RFNG
Alternative Names
RFNG antibody; Beta-1,3-N-acetylglucosaminyltransferase radical fringe antibody; EC 2.4.1.222 antibody; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase antibody
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Beta-1,3-N-acetylglucosaminyltransferase radical fringe protein (71-331AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RFNG Antibody (Product code: CSB-PA896922LA01HU) is Non-conjugated. For RFNG Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA896922LB01HU RFNG Antibody, HRP conjugated ELISA
FITC CSB-PA896922LC01HU RFNG Antibody, FITC conjugated
Biotin CSB-PA896922LD01HU RFNG Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:500-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1. May be involved in limb formation and in neurogenesis.
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 31 family
Database Links

HGNC: 9974

OMIM: 602578

KEGG: hsa:5986

STRING: 9606.ENSP00000307971

UniGene: Hs.569700

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