RFWD2 Antibody

Code CSB-PA019607GA01HU
Size $600
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Product Details

Uniprot No.
Target Names
RFWD2
Alternative Names
COP1; RFWD2; RNF200; E3 ubiquitin-protein ligase COP1; Constitutive photomorphogenesis protein 1 homolog; hCOP1; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-type E3 ubiquitin transferase RFWD2
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Human RFWD2
Immunogen Species
Homo sapiens (Human)
Isotype
IgG
Purification Method
Antigen Affinity purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
Tested Applications
ELISA,WB,IHC
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif.
Gene References into Functions
  1. COP1 overexpression inhibits p53 expression induced by fludarabine and promotes ubiquitin-mediated p53 degradation in chronic lymphocytic leukemia cells. PMID: 30423551
  2. miR-103 blocked PI3K/AKT signal pathway by regulation of COP1. These data indicated that miR-103 was up-regulated in drug resistant cells and it may regulate ADR-resistance by regulation of COP1 in AML cells. PMID: 29058777
  3. COP1 regulates human breast cancer cell proliferation and apoptosis in a p53-dependent manner.The COP1-mediated degradation of p53 regulates cancer cell growth and apoptosis. PMID: 29516369
  4. that COP1 may play a role in promoting glioma cell proliferation by interacting with and downregulating tumor suppressor p53 rather than oncogenic protein c-JUN PMID: 27534417
  5. COP1 forms complex with p53 protein and plays a role in p53 down-regulation. PMID: 29379285
  6. Authors demonstrate that mtp53 prevents the COP1/DET1 complex from ubiquitinating ETS2 and thereby marking it for destruction. Authors show that mtp53 destabilizes DET1 and also disrupts the DET1/ETS2 complex thereby preventing ETS2 degradation. PMID: 26871468
  7. STK40 binds the COP1 WD40 domain using a VPD/E motif in its C-terminal tail. PMID: 28089446
  8. In conclusion, miR-214 functions as a tumor suppressor by regulating the RFWD2-p53 cascade, thus delivery of miR-214 analogs could be a potential adjunct therapy in breast cancer harboring wild type p53. PMID: 27422604
  9. the reduced expression of COP1 and the upregulated expression of ETV1 in RCC tissue samples, which was associated with a high tumor-node-metastasis stage of RCC. Furthermore, the overexpression of COP1 in the RCC ACHN cells inhibited the migration and invasion of ACHN cells, and downregulated ETV1 and MMP7 expression levels. PMID: 27278120
  10. COP1 expression was an independent predictor of overall survival. PMID: 26753957
  11. protein level changes lead to increased sensitivity toward cisplatin treatment, implicating that huCOP1 plays a positive role in maintaining genome integrity in human keratinocytes. PMID: 27995412
  12. the present study revealed that COP1 plays an important role in CLL cell proliferation and tumorigenicity, and may be a useful indicator of the chronic lymphocytic leukemia processes. PMID: 26717976
  13. COP1 directly interacts with p27 through a VP motif on p27 and functions as an E3 ligase of p27 to accelerate the ubiquitin-mediated degradation of p27. COP1-p27 axis deregulation is involved in tumorigenesis. PMID: 26254224
  14. COP1 overexpression leads to the cytoplasmic distribution of p27, thereby accelerating p27 degradation. PMID: 25945542
  15. COP1 negatively regulates ETV1 in patients with triple-negative breast cancer. PMID: 25884720
  16. changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes upon UVB irradiation PMID: 25169772
  17. Phosphorylation of the ETS1 and ETS2 transcriptional oncoproteins at specific serine or threonine residues creates binding sites for the COP1 tumor suppressor protein. PMID: 25117710
  18. while the role of COP1 in malignancies is controversial, our current data support that COP1 acts as a tumor suppressor in gastric cancer. PMID: 23933908
  19. co-expressing COP1 and active GSK3beta blocked in vitro cell growth/migration and in vivo metastasis of invasive breast cancer cells. PMID: 24027432
  20. COP1 physically interacted with PTP1B and suppressed PTP1B phosphatase activity as well as the association of PTP1B with IRbeta. PMID: 23439647
  21. Modulation of fatty acid synthase degradation by concerted action of p38 MAP kinase, E3 ligase COP1, and SH2-tyrosine phosphatase Shp2. PMID: 23269672
  22. High level of COP1 expression is associated with poor prognosis in primary gastric cancer. PMID: 23091414
  23. Data define the subcellular localization and regulation of COP1 after DNA damage and provide a mechanistic explanation for the notion that 14-3-3sigma's impact on the inhibition of p53 E3 ligases is an important step for p53 stabilization after DNA damage. PMID: 20843328
  24. data suggest that the CSN6-COP1 axis is involved in 14-3-3sigma degradation, and that deregulation of this axis will promote cell growth and tumorigenicity PMID: 21625211
  25. the ubiquitin ligase COP1 (also known as RFWD2) is a tumour suppressor that negatively regulates ETV1, ETV4 and ETV5; ETV1, which is mutated in prostate cancer more often, was degraded after being ubiquitinated by COP1 PMID: 21572435
  26. MDM2, MDMX, Pirh2 and COP1 might inhibit p53 activity synergistically in vivo. PMID: 20333547
  27. RFWD2 is associated with acute lung injury in mice PMID: 21297076
  28. Increased COP1 is associated with hepatocellular carcinoma. PMID: 20959491
  29. COP1 contributes to UVB-induced signaling in human keratinocytes PMID: 19741714
  30. DET1 promotes ubiquitination and degradation of c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1 PMID: 14739464
  31. tumour-suppressor protein p53 is a COP1-interacting protein; COP1 is a critical negative regulator of p53 PMID: 15103385
  32. Results suggest that overexpression of COP1 contributes to the accelerated degradation of p53 protein in cancers and attenuates the tumor suppressor function of p53. PMID: 15492238
  33. in response to DNA damage, ATM phosphorylated COP1 on Ser(387) and stimulated a rapid autodegradation mechanism; ionizing radiation triggered an ATM-dependent movement of COP1 from the nucleus to the cytoplasm PMID: 16931761
  34. dynamic changes of the COP1/COP1D ratio provide an additional level of regulation of the half-life of the substrates of this E3 ligase under homeostatic or pathological conditions PMID: 17968316
  35. COP1 binds FoxO1, enhances its ubiquitination, and promotes its degradation via the ubiquitin-proteasome pathway. PMID: 18815134
  36. Disruption of the COP1-mediated proteolysis by ionizing radiation leads to MTA1 stabilization. PMID: 19805145
  37. The ubiquitin ligase COP1 is a critical negative regulator of p53 and transcriptionally inducible by p53. PMID: 15103385

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Subcellular Location
Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles.
Protein Families
COP1 family
Tissue Specificity
Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
Database Links

HGNC: 17440

OMIM: 608067

KEGG: hsa:64326

STRING: 9606.ENSP00000356641

UniGene: Hs.523744

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