RFXANK Antibody

Datasheet

Code	CSB-PA019617LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate All lanes: RFXANK antibody at 6.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 25, 26 kDa Observed band size: 29 kDa IHC image of CSB-PA019617LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of PC-3 cells with CSB-PA019617LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RFXANK Polyclonal antibody

Uniprot No.

O14593

Target Names

RFXANK

Alternative Names

ANKRA1 antibody; Ankyrin repeat containing regulatory factor X associated protein antibody; Ankyrin repeat family A protein 1 antibody; BLS antibody; DNA-binding protein RFXANK antibody; F14150_1 antibody; MGC138628 antibody; Regulatory factor X associated ankyrin containing protein antibody; Regulatory factor X subunit B antibody; Regulatory factor X, ankyrin repeat containing antibody; Regulatory factor X-associated ankyrin-containing protein antibody; RFX Bdelta4 antibody; RFX-B antibody; RFXANK antibody; RFXB antibody; RFXB delta 4 antibody; RFXK_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DNA-binding protein RFXANK protein (3-84AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RFXANK Antibody (Product code: CSB-PA019617LA01HU) is Non-conjugated. For RFXANK Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA019617LB01HU	RFXANK Antibody, HRP conjugated	ELISA
FITC	CSB-PA019617LC01HU	RFXANK Antibody, FITC conjugated
Biotin	CSB-PA019617LD01HU	RFXANK Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. May also potentiate the activation of RAF1.; Isoform 2 is not involved in the positive regulation of MHC class II genes.

Gene References into Functions

mutation results in Bare Lymphocyte Syndrome, Type 2 in a child of Mexican descent PMID: 26634365
genetic, clinical, and immunologic features of 35 patients from 30 unrelated kindreds from North Africa sharing the same RFXANK founder mutation, a 26-bp deletion called I5E6-25_I5E6 + 1) PMID: 21908431
Findings confirm the association between the high frequency of the combined immunodeficiency and the defect in MHC class II expression and provides strong evidence for a founder effect of the 752delG26 mutation in the North African population. PMID: 20414676
Low levels of RFX-B are found in macrophages of colorectal cancer patients, partly explaining immunodeficiency in cancer. PMID: 11836625
in vivo effects of mutations on the expression of the RFXANK RNA and protein PMID: 12618906
ANKRA, RFXANK, and CIITA are novel targets of class IIa HDACs which may deacetylases play a role in regulating MHCII expression PMID: 15964851
analysis of RFXANK domains and function PMID: 16166641
RFXAP and RFXB have roles in relieving autoinhibition of RFX5 PMID: 18723135

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Involvement in disease

Bare lymphocyte syndrome 2 (BLS2)

Subcellular Location

Cytoplasm. Nucleus.

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 9987

OMIM: 209920

KEGG: hsa:8625

STRING: 9606.ENSP00000305071

UniGene: Hs.153629

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Antigen processing and presentation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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