RHOF Antibody

Code CSB-PA867192LA01HU
Size US$166
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Image
  • Western blot
    All lanes: RHOF antibody at 3µg/ml
    Lane 1: Colo320 whole cell lysate
    Lane 2: RAW264.7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 24, 19 kDa
    Observed band size: 24 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA867192LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA867192LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RHOF Polyclonal antibody
Uniprot No.
Target Names
RHOF
Alternative Names
ARHF antibody; FLJ20247 antibody; Ras homolog gene family member F (in filopodia) antibody; Ras homolog gene family member F antibody; Rho F antibody; Rho family GTPase Rif antibody; Rho in filopodia antibody; Rho related GTP binding protein RhoF antibody; Rho-related GTP-binding protein RhoF antibody; Rhof antibody; RHOF_HUMAN antibody; RIF antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Rho-related GTP-binding protein RhoF protein (1-170AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RHOF Antibody (Product code: CSB-PA867192LA01HU) is Non-conjugated. For RHOF Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA867192LB01HU RHOF Antibody, HRP conjugated ELISA
FITC CSB-PA867192LC01HU RHOF Antibody, FITC conjugated
Biotin CSB-PA867192LD01HU RHOF Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Gene References into Functions
  1. Rif has two separate roles in FARP1 regulation-in controlling its association with plexinA4, and in releasing active RhoA from a plexinA4/FARP1 complex. PMID: 25668492
  2. In the present review, our aim is to summarize the current information about the Rif effectors and how Rif remodels actin cytoskeleton in many aspects. [Review] PMID: 22260703
  3. The authors show that enteropathogenic Escherichia coli translocates EspH, which inactivates mammalian RhoGEFs and triggers cytotoxicity. PMID: 22251971
  4. Rif GTPase is an alternative trigger of stress-fiber formation in epithelial cells. PMID: 20233848
  5. deletion of genes RHOF in early-stage mycosis fungoides. PMID: 18663754
  6. The protein encoded by this gene causes the formation of long, actin-rich filopodia. In combination with RAC1 and CDC42 it causes additional actin rearrangments, suggesting that this protein is intimately involved in regulation of the cytoskeleton. PMID: 11084341

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton.
Protein Families
Small GTPase superfamily, Rho family
Database Links

HGNC: 15703

KEGG: hsa:54509

STRING: 9606.ENSP00000267205

UniGene: Hs.707579

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