RHOF Antibody

Datasheet

Code	CSB-PA867192LA01HU
Size	US$166
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Image	Western blot All lanes: RHOF antibody at 3µg/ml Lane 1: Colo320 whole cell lysate Lane 2: RAW264.7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 24, 19 kDa Observed band size: 24 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA867192LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA867192LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RHOF Polyclonal antibody

Uniprot No.

Q9HBH0

Target Names

RHOF

Alternative Names

ARHF antibody; FLJ20247 antibody; Ras homolog gene family member F (in filopodia) antibody; Ras homolog gene family member F antibody; Rho F antibody; Rho family GTPase Rif antibody; Rho in filopodia antibody; Rho related GTP binding protein RhoF antibody; Rho-related GTP-binding protein RhoF antibody; Rhof antibody; RHOF_HUMAN antibody; RIF antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Rho-related GTP-binding protein RhoF protein (1-170AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RHOF Antibody (Product code: CSB-PA867192LA01HU) is Non-conjugated. For RHOF Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA867192LB01HU	RHOF Antibody, HRP conjugated	ELISA
FITC	CSB-PA867192LC01HU	RHOF Antibody, FITC conjugated
Biotin	CSB-PA867192LD01HU	RHOF Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.

Gene References into Functions

Rif has two separate roles in FARP1 regulation-in controlling its association with plexinA4, and in releasing active RhoA from a plexinA4/FARP1 complex. PMID: 25668492
In the present review, our aim is to summarize the current information about the Rif effectors and how Rif remodels actin cytoskeleton in many aspects. [Review] PMID: 22260703
The authors show that enteropathogenic Escherichia coli translocates EspH, which inactivates mammalian RhoGEFs and triggers cytotoxicity. PMID: 22251971
Rif GTPase is an alternative trigger of stress-fiber formation in epithelial cells. PMID: 20233848
deletion of genes RHOF in early-stage mycosis fungoides. PMID: 18663754
The protein encoded by this gene causes the formation of long, actin-rich filopodia. In combination with RAC1 and CDC42 it causes additional actin rearrangments, suggesting that this protein is intimately involved in regulation of the cytoskeleton. PMID: 11084341

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Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton.

Protein Families

Small GTPase superfamily, Rho family

Database Links

HGNC: 15703

KEGG: hsa:54509

STRING: 9606.ENSP00000267205

UniGene: Hs.707579

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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