RPL7A Antibody

Datasheet

Code	CSB-PA020303LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate All lanes: RPL7A antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa IHC image of CSB-PA020303LA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RPL7A Polyclonal antibody

Uniprot No.

P62424

Target Names

RPL7A

Alternative Names

60S ribosomal protein L7a antibody; L7A antibody; MGC103146 antibody; PLA X polypeptide antibody; PLA-X polypeptide antibody; ribosomal protein L7a antibody; RL7A_HUMAN antibody; RP23-414L19.4 antibody; RPL7A antibody; SURF 3 antibody; SURF-3 antibody; SURF3 antibody; surfeit 3 antibody; Surfeit locus protein 3 antibody; Thyroid hormone receptor uncoupling protein antibody; TRUP antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human 60S ribosomal protein L7a protein (2-138AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RPL7A Antibody (Product code: CSB-PA020303LA01HU) is Non-conjugated. For RPL7A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA020303LB01HU	RPL7A Antibody, HRP conjugated	ELISA
FITC	CSB-PA020303LC01HU	RPL7A Antibody, FITC conjugated
Biotin	CSB-PA020303LD01HU	RPL7A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

X-ray diffraction data of RPL7a were collected to a resolution of 3.5 A from a crystal belonging to the tetragonal space group P4(1)22 or P4(3)22 with unit-cell parameters a = 92.28, b = 92.28, c = 236.59 A PMID: 21505254
ethanol-induced alteration in expression may mediate the promoting effects of ethanol on breast cancer development PMID: 11759826
the L7a protein contains two RNA-binding domains: one encompassing aa 52-100 (RNAB1) and the other encompassing aa 101-161 (RNAB2) PMID: 15361074
Under-expression of RPL7A may be involved in the carcinogenesis of osteosarcoma PMID: 19125294

Involvement in disease

Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene.

Protein Families

Eukaryotic ribosomal protein eL8 family

Database Links

HGNC: 10364

OMIM: 185640

KEGG: hsa:6130

STRING: 9606.ENSP00000361076

UniGene: Hs.499839

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Protein Service

Gene Synthesis Service

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The Overview of Organelles Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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