RPL7A Antibody

Datasheet
Code CSB-PA020303LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. P62424
Image
  • Western Blot
    Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate
    All lanes: RPL7A antibody at 5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • IHC image of CSB-PA020303LA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human 60S ribosomal protein L7a protein (2-138AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance cytoplasm, cytosol, cytosolic large ribosomal subunit, extracellular exosome, focal adhesion, membrane, nucleolus, nucleus, polysomal ribosome, cadherin binding
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias 60S ribosomal protein L7a (Large ribosomal subunit protein eL8) (PLA-X polypeptide) (Surfeit locus protein 3), RPL7A, SURF-3 SURF3
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names RPL7A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Involvement in disease Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene.
Protein Families Eukaryotic ribosomal protein eL8 family
Database Links

HGNC: 10364

OMIM: 185640

KEGG: hsa:6130

STRING: 9606.ENSP00000361076

UniGene: Hs.499839

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