RPS3 Antibody

Code CSB-PA004002
Size US$100
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  • Western Blot analysis of HepG2 cells using Ribosomal Protein S3 Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
RPS3
Alternative Names
40S ribosomal protein S3 antibody; fb13d09 antibody; FLJ26283 antibody; FLJ27450 antibody; IMR 90 ribosomal protein S3 antibody; MGC56088 antibody; MGC87870 antibody; OTTHUMP00000229804 antibody; OTTHUMP00000229805 antibody; OTTHUMP00000229874 antibody; OTTHUMP00000229877 antibody; OTTHUMP00000229878 antibody; OTTHUMP00000229879 antibody; OTTHUMP00000229880 antibody; OTTHUMP00000229882 antibody; OTTHUMP00000229883 antibody; OTTHUMP00000229886 antibody; Ribosomal protein S3 antibody; rps3 antibody; RS3_HUMAN antibody; S3 antibody; wu:fb13d09 antibody; zgc:56088 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthesized peptide derived from the C-terminal region of Human Ribosomal Protein S3.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Involved in translation as a component of the 40S small ribosomal subunit. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS). Has also been shown to bind with similar affinity to intact and damaged DNA. Stimulates the N-glycosylase activity of the base excision protein OGG1. Enhances the uracil excision activity of UNG1. Also stimulates the cleavage of the phosphodiester backbone by APEX1. When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage. Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide. Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes. Represses its own translation by binding to its cognate mRNA. Binds to and protects TP53/p53 from MDM2-mediated ubiquitination. Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization. Involved in induction of apoptosis through its role in activation of CASP8. Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5. Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation.
Gene References into Functions
  1. Results found that BfrB subverts the host innate immune system by binding the NF-kappaB subunit RPS3 and promotes the survival of mycobacteria in macrophages by inhibiting cytokine production in host cells. PMID: 29018126
  2. These results reveal that RPS3 upregulates XIAP independently of the NF-kappaB pathway in human breast cancer cells PMID: 29048653
  3. Asn 165 residue of rpS3 is a critical site for N-linked glycosylation and passage through the ER-Golgi secretion pathway. PMID: 27384988
  4. findings suggest that uS3 residing in the 40S ribosome might perform extra-ribosomal functions related to control of DNA quality PMID: 28334742
  5. Short 5'UTR mRNAs are enriched with TISU (translation initiator of short 5'UTR), a 12-nucleotide element directing efficient scanning-independent translation. This study demonstrate that TISU is particularly dependent on eukaryotic initiation factor 1A (eIF1A) which interacts with both RPS3 and RPS10e. PMID: 28584194
  6. Data show that ribosomal protein S3 (RPS3) knockdown decreased mitochondrial calcium uptake 1 protein (MICU1) expression. PMID: 26336993
  7. Increased RPS3 expression is associated with osteosarcoma invasion. PMID: 25449781
  8. RPS3, a component of basic translation machinery operates at initiation and most probably elongation of protein synthesis, is also implicated in various events of the cell life as an extraribosomal player. [Review] PMID: 24239944
  9. These findings suggest that the secreted rpS3 protein is an indicator of malignant tumors. PMID: 24211576
  10. rpS3 accumulates in the mitochondria to repair damaged DNA due to the decreased interaction between rpS3 and HSP90 in the cytosol. PMID: 23911537
  11. rpS3 acts as a microtubule associated protein and regulates spindle dynamics during mitosis. PMID: 23131551
  12. A novel radioresistance mechanism through functional orchestration of rpS3, TRAF2, and NF-kappaB in non-small cell lung cancer cells, is reported. PMID: 23188828
  13. rpS3 is recruited to the DISC and plays a critical role in both genotoxic stress and cytokine induced apoptosis. PMID: 22510408
  14. The phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase. PMID: 21871177
  15. rpS3 is covalently modified by SUMO-1 and this post-translational modification regulates rpS3 function by increasing rpS3 protein stability. PMID: 21968017
  16. Data show that the IKKbeta-dependent modification of a specific amino acid in RPS3 promoted specific NF-kappaB functions that underlie the molecular pathogenetic mechanisms of E. coli O157:H7. PMID: 21399639
  17. PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-kappaB and MAP kinase, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation. PMID: 20709134
  18. when Flag-tagged rpS3 was transiently transfected into 293T cells, the level of endogenous rpS3 gradually decreased regardless of transcription PMID: 20217897
  19. DNA pull-down assays using a 7,8-dihydro-8-oxoguanine duplex oligonucleotide as a substrate found that RPS3 acted as a scaffold for the additional binding of MDM2 and p53. PMID: 19656744
  20. Electron paramagnetic resonance study reveals a putative iron-sulfur cluster in human rpS3 protein. PMID: 11911468
  21. RPS3 is involved in apoptosis. PMID: 14988002
  22. Using surface plasmon resonance technology, the authors show that ribosomal protein S3 positively interacts with the human base excision repair enzymes N-glycosylase/apurinic-apyrimidinic lyase OGG1 and APE/Ref-1. PMID: 15518571
  23. The S3-K132A mutant retained the ability to cleave abasic DNA, but its capacity to bind 8-oxoG was abrogated completely. PMID: 16737853
  24. PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer PMID: 17140567
  25. S3 is a key protein at the mRNA binding site neighboring mRNA downstream of the codon at the decoding site in the human ribosome. PMID: 17179743
  26. hRpS3 may be involved in the uracil-excision pathway, probably by participating in the DNA repair mechanism to remove uracil generated by the deamination of cytosine in DNA, and by preventing C/G-->T/A transition mutations. PMID: 18973764
  27. Protein S3 fragments neighboring mRNA during elongation and translation termination on the human ribosome PMID: 19088750
  28. these results clearly show that arginine methylation of rpS3 plays a critical role in its import into the nucleolus, as well as in small subunit assembly of the ribosome. PMID: 19460357

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Subcellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus. Mitochondrion inner membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton, spindle.
Protein Families
Universal ribosomal protein uS3 family
Database Links

HGNC: 10420

OMIM: 600454

KEGG: hsa:6188

STRING: 9606.ENSP00000433821

UniGene: Hs.546286

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