RUFY3 Antibody

Datasheet

Code	CSB-PA765974LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: RUFY3 antibody at 3.71µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 56, 71, 65 kDa Observed band size: 53 kDa Immunofluorescence staining of Hela cells with CSB-PA765974LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RUFY3 Polyclonal antibody

Uniprot No.

Q7L099

Target Names

RUFY3

Alternative Names

Protein RUFY3 antibody; rap2 interacting protein x antibody; Rap2 interacting protein x isoform 2 antibody; Rap2-interacting protein x antibody; RIPx antibody; Rpipx antibody; Rufy3 antibody; RUFY3_HUMAN antibody; RUN and FYVE domain containing 3 antibody; Singar antibody; Singar1 antibody; Single axon regulated protein antibody; single axon related 1 antibody; Single axon-regulated protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein RUFY3 protein (243-352AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RUFY3 Antibody (Product code: CSB-PA765974LA01HU) is Non-conjugated. For RUFY3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA765974LB01HU	RUFY3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA765974LC01HU	RUFY3 Antibody, FITC conjugated
Biotin	CSB-PA765974LD01HU	RUFY3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the generation of neuronal polarity formation and axon growth. Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes. Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery. Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones. Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner.

Gene References into Functions

these findings provide important evidence that PAK1 can positively regulate RUFY3 expression, which contribute to the metastatic potential of gastric cancer cells PMID: 25766321
Rab5(Q79L) interacts with the carboxyl terminus of RUFY3 PMID: 20376209

Subcellular Location

Cytoplasm. Endomembrane system. Cell projection, invadopodium. Perikaryon. Cell projection. Cell projection, growth cone. Cell projection, filopodium. Cell projection, lamellipodium.

Tissue Specificity

Overexpressed in gastric cancer cells and tissues (at protein level).

Database Links

HGNC: 30285

OMIM: 611194

KEGG: hsa:22902

STRING: 9606.ENSP00000370394

UniGene: Hs.740904

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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