Code | CSB-PA120525 |
Size | US$166 |
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Application | Recommended Dilution |
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ELISA | 1:1000-1:2000 |
IHC | 1:25-1:100 |
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FortheproductCSB-PA120525, I would like to know the type of fixative solution. We have already bought this antibody from your company. We want to try IHC using this antibody.
1) Antigen retrieval solution:
We would suggest using sodium citrate buffer solution (500ml) with a combination of high-temperature heating and pressure cooking for the antigen retrieval in IHC, please check the detailed antigen retrieval protocol below:
1. 3% H2O2 is used to remove the interference of endogenous enzyme for 5min.
2. Soak slices in PBS pH 7.4.
3. Prepare 500ml citric acid sodium citrate buffer in a 1L beaker, and place it in a pressure cooker. Heat the buffer with a gasket in the water bath to 100℃.
Then, the staining rack is placed in the boiled buffer. The beaker mouth is sealed with plastic wrap to prevent the water vapor from entering the buffer during heating.
Continue heating the cooker with 1000W power to a high-pressure state and keep it for 2min. Then, the heating is stopped immediately.
4. Wait until the pressure cooker cools down and open the lid. Take out the beaker and cool it to room temperature by natural cooling.
5. Wash the slices with PBS for 5min by 3 times.
2) Type of fixative solution:
It is 2%APES-Acetone solution fixation
3) In view of the ordering timeline, we realize that the antibodies you ordered may have expired, please double-confirm.