SCUBE2 Antibody

Datasheet

Code	CSB-PA865101LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HT29 whole cell lysate, Rat spleen tissue All lanes: SCUBE2 antibody at 8.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 110, 107, 89 kDa Observed band size: 110, 89 kDa IHC image of CSB-PA865101LA01HU diluted at 1:400 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SCUBE2 Polyclonal antibody

Uniprot No.

Q9NQ36

Target Names

SCUBE2

Alternative Names

4932442O19Rik antibody; Cegb1 antibody; Cegf1 antibody; CEGP1 antibody; Cegp1 protein antibody; CUB domain and EGF-like repeat containing 1 antibody; FLJ16792 antibody; FLJ35234 antibody; ICRFP703B1614Q5.1 antibody; MGC133057 antibody; Protein CEGP1 antibody; RGD1563998 antibody; SCUB2_HUMAN antibody; Scube2 antibody; Signal peptide; CUB and EGF-like domain-containing protein 2 antibody; Signal peptide; CUB domain; EGF-like 2 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Signal peptide, CUB and EGF-like domain-containing protein 2 protein (534-703AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SCUBE2 Antibody (Product code: CSB-PA865101LA01HU) is Non-conjugated. For SCUBE2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA865101LB01HU	SCUBE2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA865101LC01HU	SCUBE2 Antibody, FITC conjugated
Biotin	CSB-PA865101LD01HU	SCUBE2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane. Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface. Synergizes with DISP1 to increase SHH secretion. Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis.

Gene References into Functions

Overexpression of SCUBE2 inhibits non-small cell lung cancer cell proliferation and invasion through suppressing the Sonic hedgehog signaling pathway. PMID: 29883759
Results confirm that heparan sulfate (HS) proteoglycans enrich Scube2 at the surface of Shh-producing cells and that Scube2-regulated proteolytic Shh processing and release depends on specific HS. PMID: 27199253
Endothelial SCUBE2 may be a novel coreceptor for VEGFR2 and potentiate VEGF-induced signaling in adult angiogenesis. PMID: 27834687
The Sonic hedgehog (Shh) signaling pathway was involved in the inhibitory effect of SCUBE2 overexpression on glioma cells. PMID: 27697090
Overexpression of SCUBE2 is associated with Breast Cancer Recurrence. PMID: 27165221
SCUBE2 expression was decreased at the transcriptional and translational levels in colorectal cancer tissues and significantly associated with clinical stage, the depth of tumor invasion, lymphnode metastasis, distant metastasis and histological grade. PMID: 25672935
Sheddases and Scube2 act cooperatively to increase the pool of soluble bioactive Shh. PMID: 24522195
Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition. PMID: 24213532
data suggest that signal peptide CUB domain (SCUBE2), like in breast cancer, associates with estrogen receptor alpha and might have a potential as prognostic or predictive marker in endometrial cancer PMID: 24053619
Via obligate assistance of Scube1 and 3, Scube2 plays a center role to recruit dually lipid-modified Hedgehog transferred from Dispatched A (DispA), increasing Hh secretion by promoting its solubility. PMID: 24084593
Domain and functional analysis of a novel breast tumor suppressor protein, SCUBE2. PMID: 21652720
identification as secreted protein that is expressed in vascular endothelium and may play important roles in development, inflammation, and thrombosis PMID: 12270931
Altered SCUBE2 expression is important in breast cancer progression and SCUBE2 may serve as a useful prognostic marker. PMID: 19369267
The full-length cDNA of human SCUBE2 was isolated and the role in the hedgehog signalling cascade, was studied. PMID: 19480626

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Subcellular Location

Secreted. Cell surface.

Tissue Specificity

Expressed in a broad spectrum of adult tissues.

Database Links

HGNC: 30425

OMIM: 611747

KEGG: hsa:57758

UniGene: Hs.523468

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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