SPPL2B Antibody

Datasheet

Code	CSB-PA823457LA01HU
Size	US$166
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Image	Immunofluorescence staining of A549 cells with CSB-PA823457LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SPPL2B Polyclonal antibody

Uniprot No.

Q8TCT7

Target Names

SPPL2B

Alternative Names

SPPL2B antibody; IMP4 antibody; KIAA1532 antibody; PSL1 antibody; Signal peptide peptidase-like 2B antibody; SPP-like 2B antibody; SPPL2b antibody; EC 3.4.23.- antibody; Intramembrane protease 4 antibody; IMP-4 antibody; Presenilin homologous protein 4 antibody; PSH4 antibody; Presenilin-like protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Signal peptide peptidase-like 2B protein (492-592AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SPPL2B Antibody (Product code: CSB-PA823457LA01HU) is Non-conjugated. For SPPL2B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA823457LB01HU	SPPL2B Antibody, HRP conjugated	ELISA
FITC	CSB-PA823457LC01HU	SPPL2B Antibody, FITC conjugated
Biotin	CSB-PA823457LD01HU	SPPL2B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus processed leader peptide gp18 of the envelope glycoprotein gp130 dependently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis.

Gene References into Functions

TfR1 N-terminal fragment is a substrate for intramembrane proteolysis by SPPL2b. PMID: 23384347
The alpha-helical content of the transmembrane domain of the British dementia protein-2 (Bri2) determines its processing by signal peptide peptidase-like 2b (SPPL2b). PMID: 22194595
SPP, SPPL2a, -2b, -2c, and -3 probably cleave type II-oriented substrate peptides as shown by consensus analysis PMID: 15385547
SPPL2b is targeted through the secretory pathway to endosomes/lysosomes, whereas SPP and SPPL3 are restricted to the ER. PMID: 15998642
Results demonstrate that SPPL2b utilizes multiple intramembrane cleavages to liberate the intracellular domain of tumor necrosis factor alpha into the cytosol. PMID: 16829951
SPPL2a and SPPL2b mediate the intramembrane cleavage, whereas neither SPP nor SPPL3 is capable of processing the Bri2 N-terminal fragment. PMID: 17965014
Alzheimer disease-like mutant SPPL2b slowed intramembrane proteolysis of tumor necrosis factor alpha and caused a relative increase of longer intracellular cleavage products. PMID: 18768471

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein; Lumenal side.

Protein Families

Peptidase A22B family

Tissue Specificity

Expressed predominantly in adrenal cortex and mammary gland.

Database Links

HGNC: 30627

OMIM: 608239

KEGG: hsa:56928

UniGene: Hs.744026

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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