ST5 Antibody

Datasheet

Code	CSB-PA022758LA01HU
Size	US$166
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Image	IHC image of CSB-PA022758LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western Blot Positive WB detected in: HepG2 whole cell lysate, U87 whole cell lysate All lanes: ST5 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 127, 83, 71 kDa Observed band size: 127 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ST5 Polyclonal antibody

Uniprot No.

P78524

Target Names

ST5

Alternative Names

DENND2B antibody; DENN domain containing protein 2B antibody; DENN domain-containing protein 2B antibody; DENN/MADD domain containing 2B antibody; HeLa tumor suppression 1 antibody; HTS1 antibody; p126 antibody; ST 5 antibody; ST5 antibody; ST5_HUMAN antibody; Suppression of tumorigenicity 5 antibody; Suppression of tumorigenicity 5 protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Suppression of tumorigenicity 5 protein (3-280AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ST5 Antibody (Product code: CSB-PA022758LA01HU) is Non-conjugated. For ST5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA022758LB01HU	ST5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA022758LC01HU	ST5 Antibody, FITC conjugated
Biotin	CSB-PA022758LD01HU	ST5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May be involved in cytoskeletal organization and tumorogenicity. Seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Plays a role in EGFR trafficking from recycling endosomes back to the cell membrane.; Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.; May block ERK2 activation stimulated by ABL1 (Probable). May alter cell morphology and cell growth (Probable).

Gene References into Functions

Here, we discover that intersectin-s binds DENND2B, a guanine nucleotide exchange factor for the exocytic GTPase Rab13, and this interaction promotes recycling of ligand-free EGFR to the cell surface. Our study thus reveals a novel mechanism controlling the fate of internalized EGFR with important implications for cancer. PMID: 29030480
DENND2B interacts with the Rab13 effector MICAL-L2 at the cell periphery, and this interaction is required for the dynamic remodeling of the cell's leading edge. PMID: 25713415
Using RNA in situ hybridisation in mouse, the authors found that St5 is expressed in the frontal cortex during embryonic development. In adult mouse brain, expression of St5 was especially high in the hippocampal area and cerebellum. PMID: 19843505

Subcellular Location

[Isoform 1]: Cytoplasm, cell cortex. Cell membrane. Recycling endosome.

Tissue Specificity

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly

Database Links

HGNC: 11350

OMIM: 140750

KEGG: hsa:6764

STRING: 9606.ENSP00000319678

UniGene: Hs.117715

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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